Journal ArticleDOI
Recommended Procedures for testing Genetic Hazards from Chemicals, based on the Induction of Dominant Lethal Mutations in Mammals
Samuel S. Epstein,G. Röhrborn +1 more
Reads0
Chats0
TLDR
This communication recommends practical procedures for the dominant lethal assay, and indicates how these can be integrated in routine toxicological practice.Abstract:
GENETIC hazards from drugs and chemical pollutants are now widely recognized and appropriate, sensitive and economic mammalian methods for detecting and measuring mutagenic effects of chemicals have been developed. These methods are relevant to man1 and include in vivo cytogenetics, host-mediated assay, and dominant lethal assay; sub-mammalian systems are generally considered to be of ancillary value only. The Advisory Panel on Mutagenicity of Pesticides1 and the Food and Drug Administration Advisory Committee on Protocols for Safety Evaluation2 have recommended that pesticides and food additives should be tested for mutagenicity in mammalian systems before registration. This communication recommends practical procedures for the dominant lethal assay, and indicates how these can be integrated in routine toxicological practice.read more
Citations
More filters
Journal ArticleDOI
Detection of chemical mutagens by the dominant lethal assay in the mouse.
TL;DR: A total of 174 test agents, including pharmaceuticals, food additives, pesticides and organic extracts of air and water pollutants, was tested for mutagenicity in mice using the modified dominant lethal assay, with results determined by increased early fetal deaths per pregnancy and, in some instances, also indirectly by reduction in total implants per pregnancy.
Book
Male-Mediated Developmental Toxicity
TL;DR: This review of studies that use a variety of developmental endpoints, including gene mutation, chromosomal abnormal ities, spontaneous abortion, congenital abnormalities, and cancer, describes potential mechanisms, highlights new data from both laboratory and epidemiologic studies, and points out limitations of previous studies and gaps in knowledge.
Book ChapterDOI
The insulin-sensitive glucose transporter.
TL;DR: An insulin-sensitive glucose transporter is described and the regulation of glucose transport proteins are discussed and an approach to understand the effects of insulin on glucose transport in vivo is to study animals during fasting.
Journal ArticleDOI
Genetic effects of turmeric and curcumin in mice and rats
TL;DR: Incorporation of either turmeric or curcumin into diets of mice did not show significant effect on the incidence of micronucleated polychromatic erythrocytes, structural and numerical aberrations in bone-marrow chromosomes, pregnancy rate, number of live and dead embryos, total implants and mutagenic index.
Journal ArticleDOI
The advantages of drosophila for mutation studies
TL;DR: Observations on modifications and specificities in Drosophila, show at least that a particular treatment can be modified, and this may then be followed up in mammalian cells, which may provide guidance on the kind of questions that should be looked for in more laborious systems.
References
More filters
Book
Chemical Mutagens: Principles and Methods for Their Detection
TL;DR: This book discusses the development and validation of short-term assays designed to detect the mutagenic effects of environmental chemicals, and the detection of mutagens in human feces as an approach to the discovery of causes of colon cancer.
Journal ArticleDOI
Chemical mutagens in the human environment.
TL;DR: Preliminary data on the feasibility of mutagenicity testing in mice based on screening a wide variety of environmental pollutants, including carcinogens, air and water pollutants, pharmaceuticals, food additives and pesticides are presented.
Journal ArticleDOI
Ethyl methanesulfonate-induced chromosome breakage in the mouse.
TL;DR: Evidence is presented which support the concept that F 1male sterility, like F 1 male semi-sterility, has a basis of chromosome breakage, and the same dose of the chemical induced high frequencies of translocations among the F 1 offspring.