Regulation of Nitrogen Fixation in Azotobacter vinelandii OP and in an Apparently Partially Constitutive Mutant
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TLDR
Methylamine and 2-methylalanine appeared to act as co-repressors of nitrogenase in Azotobacter vinelandii OP, and a mutant of strain OP, selected on the basis of its relative resistance to methylalanine, appeared partially constitutive because nitrogen enzyme in this strain was less sensitive to repressors than was the enzyme in the wild-type strain.Abstract:
Methylamine and 2-methylalanine appeared to act as co-repressors of nitrogenase in Azotobacter vinelandii OP. They inhibited the growth of this organism on molecular nitrogen but not on nitrate, ammonia, or Casamino Acids; they prevented the formation of nitrogenase by cells transferred from repression to induction conditions; and they did not inhibit the activity of nitrogenase in vitro. A mutant of strain OP, selected on the basis of its relative resistance to methylalanine, appeared partially constitutive because nitrogenase in this strain was less sensitive to repressors than was the enzyme in the wild-type strain.read more
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Book ChapterDOI
The Family Azotobacteraceae
TL;DR: Investigations on rRNA similarities of various Gram-negative bacteria based on Two values of DNA-rRNA hybrids showed that Azotobacter and Azomonas are closely related to one another and to the Pseudomonas fluorescens rRNA branch.
Journal ArticleDOI
Environmental effects on the growth of nitrogen‐fixing bacteria
TL;DR: Studies with Azotobacter chroococcum in chemostats have clarified several aspects of the physiology of nitrogen fixation by this obligate aerobe, and respiration appeared to perform a protective function.
Journal ArticleDOI
Dinitrogen (N 2 ) fixation (with a biochemical emphasis).
H Dalton,L E Mortenson +1 more
Journal ArticleDOI
Regulation of Nitrogen Fixation in Azotobacter vinelandii OP: the Role of Nitrate Reductase
TL;DR: A number of chlorate-resistant mutants were selected, and one of these, clr68-5, was studied in detail, and it was found that this mutant cannot utilize nitrate in vivo to overcome the effect of nonmetabolizable repressors of nitrogenase.
Journal ArticleDOI
Azotobacter vinelandii: the source of 100 years of discoveries and many more to come.
TL;DR: The history and potential for future research using this versatile microbe are summarized, which includes its ability to produce multiple valuable polymers - bioplastic and alginate in particular; its nitrogen-fixing prowess; its production of potentially useful enzymes and metabolic pathways; and even its biofuel production abilities.
References
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Journal ArticleDOI
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Journal ArticleDOI
Nitrogen fixation: hydrosulfite as electron donor with cell-free preparations of azotobacter vinelandii and rhodospirillum rubrum.
TL;DR: The analyses reported in Table 1 indicate that the fragment I used by us had 7 or 8, rather than 6 disulfide bonds, leading to an even lower probability for return to the native structure by random reoxidation.
Journal ArticleDOI
Photometabolism of Rhodospirillum rubrum: light-dependent dissimilation of organic compounds to carbon dioxide and molecular hydrogen by an anaerobic citric acid cycle.
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