Journal ArticleDOI
Resolution of the fluorescence lifetimes in a heterogeneous system by phase and modulation measurements
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This article is published in The Journal of Physical Chemistry.The article was published on 1981-04-01. It has received 260 citations till now. The article focuses on the topics: Resolution (electron density) & Phase (waves).read more
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Journal ArticleDOI
The Phasor Approach to Fluorescence Lifetime Imaging Analysis
TL;DR: The phasor approach has the potential to simplify the way data are analyzed in FLIM, paving the way for the analysis of large data sets and, in general, making the FLIM technique accessible to the nonexpert in spectroscopy and data analysis.
Journal ArticleDOI
A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution
Enrico Gratton,M. Limkeman +1 more
TL;DR: A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported and some application to the measurement of multiexponential decays is illustrated.
Journal ArticleDOI
Analysis of fluorescence decay kinetics from variable-frequency phase-shift and modulation data
TL;DR: Analysis of fluorescence phase-shift and modulation data by the method of nonlinear least squares to determine the values of the lifetimes and fractional intensities for a mixture of exponentially decaying fluorophores broadly confirms the predicted resolving potential of variable-frequency phase-modulation fluorometry.
Journal ArticleDOI
The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
TL;DR: In this article, the authors quantified the extent of sample heterogeneity in a melange of fluorophores and found that the heterogeneity arises from solvent-fluorophore interactions or from the fluorophore per se.
Journal ArticleDOI
Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale
TL;DR: The frequency domain fluorescence lifetime imaging microscope (FLIM) as discussed by the authors was developed to determine the lifetime of a continuous wave laser excitation source of an epi-illumination fluorescence microscope at a high frequency fA.