A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution
Enrico Gratton,M. Limkeman +1 more
TLDR
A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported and some application to the measurement of multiexponential decays is illustrated.About:
This article is published in Biophysical Journal.The article was published on 1983-12-01 and is currently open access. It has received 498 citations till now. The article focuses on the topics: Fluorometer & Phase (waves).read more
Citations
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Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence
TL;DR: It is concluded that the presence of water molecules in the phospholipid matrix are responsible for the spectral properties of Laurdan in the gel phase.
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Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell.
TL;DR: Fluorescence lifetime imaging microscopy is a technique in which the mean fluorescence lifetime of a chromophore is measured at each spatially resolvable element of a microscope image to allow exploration of the molecular environment of labelled macromolecules in the interior of cells.
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Biomedical Photonics Handbook
TL;DR: This volume discusses biomedical photonics, spectroscopy and microscopy, the basic physical principles underlying the technology and its applications.
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Analysis of fluorescence decay kinetics from variable-frequency phase-shift and modulation data
TL;DR: Analysis of fluorescence phase-shift and modulation data by the method of nonlinear least squares to determine the values of the lifetimes and fractional intensities for a mixture of exponentially decaying fluorophores broadly confirms the predicted resolving potential of variable-frequency phase-modulation fluorometry.
References
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Measurements of Subnanosecond Fluorescence Lifetimes with a Cross-Correlation Phase Fluorometer
TL;DR: In this article, the authors considered a single fluorescent species as a detector in which the response to an infinitely narrow pulse of light decays in time, t, according to the exponential law I ( t ) = I(0) x exp (kt).
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Influence of Brownian Rotations and Energy Transfer upon the Measurements of Fluorescence Lifetime
TL;DR: In this paper, a system of first-order linear differential equations containing as only parameters the rate of fluorescence emission and rate of transport of the excitation from one orthogonal component of the emission to another is described.
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Resolution of the fluorescence lifetimes in a heterogeneous system by phase and modulation measurements
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Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometry.
TL;DR: This method is judged to provide more reliable lifetime measurements by the phase and modulation method and to eliminate artifactual photomultiplier effects in measurements of the lifetimes of NADH and indole solutions quenched by iodide.
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The Determination of the Fluorescence Lifetimes of Dissolved Substances by a Phase Shift Method
E. A. Bailey,G. K. Rollefson +1 more
TL;DR: In this article, the lifetime of fluorescent substances is determined by exciting the fluorescence with light modulated at a high frequency and measuring the difference in phase between the exciting light and the resulting fluorescence.