Role of sulA and sulB in filamentation by lon mutants of Escherichia coli K-12.
S Gottesman,E Halpern,P Trisler +2 more
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The simplest model to account for the observations is that sulA and lon participate in a pathway of filamentation independent of that which produces transient filamentation in wild-type strains; sulB product may be the target of sulA action and may play a role in normal cell division.Abstract:
Cells containing the pleiotropic Escherichia coli mutation lon filament extensively and die after exposure to ultraviolet light Outside suppressors of the ultraviolet sensitivity, called sul, have previously been described at two loci; these mutations reverse the ultraviolet sensitivity of lon strains but do not affect the mucoidal or degradation defect of these strains An isogenic set of strains carrying combinations of lon, sulA, and sulI was constructed, and their behavior during normal growth and after ultraviolet treatment was studied sulA mutations had no detectable phenotype in lon+ cells; the lon sulA strains filamented transiently after ultraviolet irradiation, as did lon+ sul+ cells We found that the sulB mutation, which alters cell morphology and slows recovery from transient filamentation after ultraviolet treatment, was epistatic to both lon and sulA Whereas sulA mutations were recessive to the wild-type allele, sulB was partially dominant The simplest model to account for our observations is that sulA and lon participate in a pathway of filamentation independent of that which produces transient filamentation in wild-type strains; sulB product may be the target of sulA action and may play a role in normal cell division Imagesread more
Citations
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Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
TL;DR: Comparing the SOS, Heat Shock, and Adaptive Regulatory Systems and evidence suggesting that UV mutagenesis does not require the induction of genes other than those repressed by LexA, the role of SOS processing to the spontaneous mutation frequency is suggested.
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The SOS regulatory system of Escherichia coli
John W. Little,David W. Mount +1 more
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Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
TL;DR: Within Escherichia coli and other species, a clear codon bias exists among the 61 amino acid codons found within the population of mRNA molecules, and the level of cognate tRNA appears directly proportional to the frequency of codon usage.
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Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor σ32
TL;DR: Evidence for the physical association of DnaK, DnaJ, and GrpE chaperones with sigma 32 in vivo is presented and distinct regulatory functions of DNAJ and Dnak/GrpE are suggested.
Journal ArticleDOI
ftsZ is an essential cell division gene in Escherichia coli.
Kang Dai,Joe Lutkenhaus +1 more
TL;DR: This strain was temperature sensitive for cell division and viability, confirming that ftsZ is an essential cell division gene in Escherichia coli and revealed that after a shift to the nonpermissive temperature, cell division ceased when the level of FtsZ started to decrease, indicating that septation is very sensitive to thelevel of FTSZ.
References
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Journal ArticleDOI
Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
TL;DR: This work states that the "SOS" Hypothesis, the Regulatory Role of DNA Damage in E. coli, and the Mechanism of SOS Repair in Bacteria and other UV-INDUCIBLE FUNCTIONS, and Regulations of SOS REPAIR, are valid hypotheses for the regulation of SOS functions.
Journal ArticleDOI
An inducible DNA replication-cell division coupling mechanism in E. coli
Olivier Huisman,Richard D'Ari +1 more
TL;DR: The work reported here establishes the existence of the latter type of replication-division coupling in E. coli, and shows that the sfiA gene product is an inducible component of this division inhibition mechanism which is synthesized at high levels after perturbations of DNA replication.
Journal ArticleDOI
A locus that controls filament formation and sensitivity to radiation in escherichia coli k-12.
TL;DR: The greater radiosensitivity of the lon mutants is not due to the loss of the capacity to reactivate uv- photoproducts in DNA because equal numbers of plaques are found when uv irradiated Tl phage is plated on lon and lon/sup +/ strains.
Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis
Miroslav Radman,L. Prakash +1 more
Journal ArticleDOI
Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif.
TL;DR: The action of tif, lon and sfi mutations is tentatively interpreted on the basis of a negative control of cell division specifically associated with DNA repair.
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