Separation of two forms of rabbit metallothionein by isoelectric focusing.
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Rabbits were given repeated injections of cadmium chloride and Cadmium- and zinc-containing protein fractions obtained from the livers of these animals showed several characteristics similar to those of the earlier described protein metallothionein.Abstract:
Rabbits were given repeated injections of cadmium chloride. Cadmium- and zinc-containing protein fractions were obtained from the livers of these animals by precipitation procedures and Sephadex G-75 chromatography. The protein thus obtained showed several characteristics similar to those of the earlier described protein metallothionein. Further separation by isoelectric focusing showed two main protein peaks with isoelectric points at 3.9 and 4.5 respectively. Amino acid analysis of these two forms showed similar content of most amino acids [residues per cent.: cysteine (28%), aspartate (8%), threonine (5-6%), serine (12%), glycine (7%), alanine (13%), methionine (2%), isoleucine (2%)] but with a small difference in content of lysine (12 and 13% respectively), proline (9 and 5% respectively) and glutamate (2 and 4% respectively). The two forms of the protein both contained cadmium, but only the one with pI4.5 contained also significant amounts of zinc.read more
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Book ChapterDOI
Chemistry and biochemistry of metallothionein.
Jeremias H. R. Kägi,Y. Kojima +1 more
TL;DR: In this review an account is given of the chemical characteristics of the 52 metallothioneins whose primary structures are now known completely or in part and of the spatial structure models derived from X-ray diffraction crystallographic analysis and two-dimensional nuclear magnetic resonance spectroscopy.
Journal ArticleDOI
Historical perspectives on cadmium toxicology
TL;DR: Findings in these studies indicate that very low exposure levels to cadmium may give rise to renal dysfunction among sensitive subgroups of human populations such as persons with diabetes.
Journal ArticleDOI
Metallothioneins and their role in the metabolism and toxicity of metals
TL;DR: The metallothioneins may also have an important role in regulating the normal absorption and homeostasis of zinc and copper as discussed by the authors, however, it is paradoxical, in that a protein synthesized within the cell to reduce toxicity, may, in itself, be toxic when excreted or leaked out from a cell to the extracellular space.
Journal ArticleDOI
Isoelectric focusing in gels
TL;DR: This review deals primarily with analytical and preparative isoelectric focusing (IEF) in gel media and reports the analysis of glycoproteins, immunoglobulins, lipoprotein, membrane proteins, peptides and metalloproteins to the study of biological systems.
Journal ArticleDOI
Equine Hepatic and Renal Metallothioneins: PURIFICATION, MOLECULAR WEIGHT, AMINO ACID COMPOSITION, AND METAL CONTENT
TL;DR: An improved, simplified, and greatly shortened isolation procedure for metallothionein from tissue extracts using gel filtration (Sephadex G-50) and DEAE-cellulose chromatography is presented.