Showing papers in "Journal of Biological Chemistry in 1960"

TL;DR: The cations of T4 phage have been examined and a balance has been obtained between total cations and total DNA anions and the replacement of the normal polyamines suggested that the polyamines may be acting as nonspecific cations.

TL;DR: The present paper outlines the phase relations of the ternary system at room temperature, gives partition coefficients of fatty acids and other materials in a standard two-phase extraction mixture, discusses use of the system in countercurrent estraction, and outlines some modifications of the original procedure that have developed during five years of analytical work in this laboratory.

TL;DR: Evidence is presented that an adenosine triphosphatase in broken human erythrocyte membranes is a part of the system for the active transport of sodium and potassium in intact ery Throthrocytes.

TL;DR: This report includes a detailed description of the method, assay results for human urine and dog adrenal vein plasma, and a critical evaluation of the specificity, precision, and accuracy of this technique.

TL;DR: It is found that free inositol may be incorporated into lipid as a result of an enzymatic exchange reaction with inositl phosphatide present in the enzyme preparation.

TL;DR: Intramolecular (nonenzymatic) condensation of the aminoaldehyde proved to be faster than the further oxidation of the aldehyde to the acid or lactam and has been developed into a rapid method for the assay of monoamine oxidase.

TL;DR: The present communication reports the results of sequence studies on each of the peptides, studies which have led to the proposed complete formula for oxidized ribonuclease given in Fig. 1.

TL;DR: In the isolation of melatonin from bovine pineal glands a chemically similar, but biologically inactive, compound was also found (Z-4), and experiments demonstrated that 0-methylation of hydroxyindoles occurs in biological reactions.

TL;DR: A theoretical consideration of the methods of evaluating the participation of the pentose cycle and the Embden-Meyerhof pathway in the metabolism of glucose and the effect of recycling of hexosemonophosphate in thepentose cycle is presented.

TL;DR: A survey of various electron acceptors revealed that oxidative phosphorylation at the firstosphorylation site was only observed if electrons passed through the rotenone-sensitive site, and both the reduction of coenzyme Q1 and the accompanying formation of adenosine triphosphate in this system are unaffected by antimycin and KCN but are almost completely abolished by roten one or Amytal.

TL;DR: The purpose of this report is to describe in detail the purification and properties of this enzyme which will be referred to as the Escherichia coli phosphodiesterase, found to hydrolyze E. coli and calf thymus DNA’s to their constituent 5’-mononucleotides once these polymers have undergone some degradation as a result either of heating or limited treatment with pancreatic DNase.

TL;DR: Results obtained with purified enzyme preparations are reported which afford a clearer insight into the nature and the sequence of reactions that occur in the biosynthesis of long chain fatty acids.

TL;DR: The new metabolite identified as N-hydroxy-2-acetylaminofluorene2, a new acidic urinary metabolite, is isolated, characterization, and synthesis and form the subject of this report.

TL;DR: Certain of the chemical, chromatographic, spectrophotometric, and nuclear magnetic properties of the substance and its derivatives that pertain to the question of structure and natural occurrence are described.

TL;DR: Methods used in the isolation of the benzimidazolylcobamide and 5,6-dimethylbenzimidazedolycobamide coenzymes, both of which have been obtained as crystalline products, are described.

TL;DR: The activities of a total of 17 enzymes that function in the Embden-Meyerhof and hexosemonophosphate oxidative pathways, the tricarboxylic acid cycle, and amino acid metabolism are studied.