Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF
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TLDR
In this paper, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777.About:
This article is published in Virology.The article was published on 1994-02-01 and is currently open access. It has received 106 citations till now. The article focuses on the topics: Porcine epidemic diarrhea virus & ORFS.read more
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Journal ArticleDOI
Identification of a new human coronavirus
Lia van der Hoek,Krzysztof Pyrc,Maarten F. Jebbink,Wilma Vermeulen-Oost,Ron J. M. Berkhout,Katja C. Wolthers,Pauline M. E. Wertheim-van Dillen,Jos Kaandorp,Joke Spaargaren,Ben Berkhout +9 more
TL;DR: A new group 1 coronavirus, HCoV-NL63, was identified in a 7-month-old child suffering from bronchiolitis and conjunctivitis as discussed by the authors.
Book ChapterDOI
The molecular biology of coronaviruses
Michael M. C. Lai,David Cavanagh +1 more
TL;DR: This chapter discusses the manipulation of clones of coronav virus and of complementary DNAs of defective-interfering RNAs to study coronavirus RNA replication, transcription, recombination, processing and transport of proteins, virion assembly, identification of cell receptors for coronaviruses, and processing of the polymerase.
Journal ArticleDOI
A previously undescribed coronavirus associated with respiratory disease in humans.
Ron A. M. Fouchier,Nico G. Hartwig,Theo M. Bestebroer,Berend Niemeyer,Jan C. de Jong,James H. M. Simon,Albert D. M. E. Osterhaus +6 more
TL;DR: The identification and characterization of a not previously recognized coronavirus obtained from an 8-mo-old boy suffering from pneumonia is described, revealing that it is most closely related to porcine epidemic diarrhea virus and HCoV 229E.
Journal ArticleDOI
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
Boyd Yount,Kristopher M. Curtis,Elizabeth A. Fritz,Lisa E. Hensley,Peter B. Jahrling,Erik Prentice,Mark R. Denison,Thomas W. Geisbert,Ralph S. Baric +8 more
TL;DR: Using a panel of contiguous cDNAs that span the entire genome, the authors have assembled a full-length cDNA of the SARS-CoV Urbani strain, and have rescued molecularly cloned SARS viruses (infectious clone SARS -CoV) that contained the expected marker mutations inserted into the component clones.
Journal ArticleDOI
Porcine epidemic diarrhea virus: An emerging and re-emerging epizootic swine virus
TL;DR: It is hoped that this review will stimulate further basic and applied studies and encourage collaboration among producers, researchers, and swine veterinarians to provide answers that improve the understanding of PEDV and PED in an effort to eliminate this economically significant viral disease.
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