SIN3 works through two different promoter elements to regulate INO1 gene expression in yeast.
TLDR
Two distinct promoter elements, the upstream repression sequence (URS1) and the InO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression.Abstract:
The SIN3 global regulatory factor affects expression of many yeast genes, including the phospholipid biosynthetic gene, INO1. Mutations in the SIN3 gene result in elevated levels of INO1 expression under conditions that normally confer full repression of INO1 transcription, indicating that SIN3 is a negative regulator of INO1. In this study, the INO1 promoter was analyzed for sequences that play a role in responding to SIN3-mediated repression. Two distinct promoter elements, the upstream repression sequence (URS1) and the INO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression.read more
Citations
More filters
Dissertation
A journey Inside Sin3 and Mi-2/ NuRD Co-repressor. Complexes: A Structural and Biochemical Perspective
TL;DR: This thesis should provide the necessary ground to understand how the PAH1 and PAH2 domain of Sin3 establish an association with their interacting partners and will help hopefully to elucidate the mechanism by which Mi-2/NuRD repress transcription.
Journal Article
Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
TL;DR: Molecular characterization of clone 583 (Acc. No. EU795363) isolated from cell suspension culture of oil palm showed that transcripts of this clone might be present in low abundance and might be a member of a multigene family in the oil palm genome.
Posted ContentDOI
Genetic screens identify multiple negative regulators that additively restrict recruitment of the SWI/SNF chromatin remodeler to the HO promoter in Saccharomyces cerevisiae
TL;DR: The results demonstrate that multiple protein complexes are important for limiting the spread of SWI/SNF mediated nucleosome eviction across the HO promoter, suggesting that regulation requires a delicate balance of activities that promote and repress transcription.
References
Related Papers (5)
INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter.
J. Ambroziak,Susan A. Henry +1 more
Repression by Ume6 Involves Recruitment of a Complex Containing Sin3 Corepressor and Rpd3 Histone Deacetylase to Target Promoters
David Kadosh,Kevin Struhl +1 more