SIN3 works through two different promoter elements to regulate INO1 gene expression in yeast.
TLDR
Two distinct promoter elements, the upstream repression sequence (URS1) and the InO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression.Abstract:
The SIN3 global regulatory factor affects expression of many yeast genes, including the phospholipid biosynthetic gene, INO1. Mutations in the SIN3 gene result in elevated levels of INO1 expression under conditions that normally confer full repression of INO1 transcription, indicating that SIN3 is a negative regulator of INO1. In this study, the INO1 promoter was analyzed for sequences that play a role in responding to SIN3-mediated repression. Two distinct promoter elements, the upstream repression sequence (URS1) and the INO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression.read more
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Journal ArticleDOI
Repression by Ume6 Involves Recruitment of a Complex Containing Sin3 Corepressor and Rpd3 Histone Deacetylase to Target Promoters
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TL;DR: It is shown that Sin3 and Rpd3 are specifically required for transcriptional repression by Ume6, a DNA-binding protein that regulates genes involved in meiosis.
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Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3.
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Journal ArticleDOI
Genetic regulation of phospholipid biosynthesis in Saccharomyces cerevisiae
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One stop mycology
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Book ChapterDOI
Genetic regulation of phospholipid metabolism: yeast as a model eukaryote.
Susan A. Henry,Jana Patton-Vogt +1 more
TL;DR: Progress over the past several decades in biochemical and molecular genetic studies of the regulation of phospholipid metabolism in baker's yeast and higher eukaryotes is discussed and a model is presented.
References
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