Journal ArticleDOI
Somatic embryogenesis and plantlet regeneration in the genus Secale : 1. Somatic embryogenesis and organogenesis from cultured immature embryos of five wild species of rye.
J. J. Rybczyński,W. Zduńczyk +1 more
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TLDR
Tissue culture of five wild species of the Secale genus found that S. africanum and S. kuprianovii appeared to have the highest embryogenic capacity among the five investigated species.Abstract:
A tissue culture of five wild species of the Secale genus, i.e., S. africanum (Stapf.), S. ancestrale (Zhuk.), S. kuprianovii (Grossh), S. segetale (Rosher.), and S. vavilovii (Grossh), from immature embryos of sizes (stages) varying between 1.0 mm to 3.0mm, cultured on MS (1962) mineral nutrient medium supplemented with 0.62 mg/1–5.0 mg/1 of 2,4-D, was established. Initially various types of callus were observed and a correlation between genotype, size of explant and 2,4-D concentration was found. The best embryogenic response was observed when explants were smaller than 1.0 mm. Induction of somatic embryogenesis of 2.0 mm–3.0 mm explants required a higher concentration of 2,4-D. Most embryoids were formed in the presence of 5.0 mg/l of 2,4-D. Secale africanum and S. kuprianovii appeared to have the highest embryogenic capacity among the five investigated species. For embryoids germination to plantlets the MS medium supplemented with GA3 and cytokinins was used. Ultimately, out of the 932 regenerants obtained 364 originated from somatic embryogenesis.read more
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Book ChapterDOI
Somatic Embryogenesis in Herbaceous Dicots
TL;DR: The seed is the primary organ for the perpetuation of germplasm and propagation in all flowering plants and contains the embryo which develops from the zygote following the fertilization of the egg cell by one of the male gametes.
Journal ArticleDOI
High Frequency of Somatic Embryogenesis and Plant Regeneration of Rye (Secale cereale L.)
Janusz Zimny,Horst Lörz +1 more
TL;DR: Following a “step by step” optimization, culture conditions have been defined giving rise to a 90—100% efficiency of somatic embryogenesis and a high number of regenerated plants.
Journal ArticleDOI
Plant regeneration from suspension culture of Iris germanica 1
TL;DR: In Iris germanica L., 'G1', 'Adorn' and 'Rococo', induction and proliferation of embryogenic calli were achieved by culture of leaf-base explants on Murashige and Skoog medium supplemented with 1 mg l−1 2,4-D, 1 mgL−1 kinetin, 200 mg l −1 casein hydrolysate, 250 mg l–1 proline and 30 g l− 1 sucrose.
Journal ArticleDOI
Somatic embryogenesis from immature inflorescences of rye.
Rosario Linacero,A. M. Vázquez +1 more
TL;DR: Evidence is provided that the genotypic effect on the in vitro response of an allogamous cultivar in rye is due to two factors: the number of competent plants and the response level of each individual.
Journal ArticleDOI
Interactions between genotypes and culture media components for improved in vitro response of rye ( Secale cereale L.) inbred lines
J. C. Popelka,F. Altpeter +1 more
TL;DR: These genotype-specific culture protocols will enable the development of efficient genetic transformation protocols for rye, maximising the plant regeneration response from rye tissue culture.
References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Somatic Embryogenesis and Plant Regeneration from Cultured Immature Embryos of Rye (Secale cereale L.)
TL;DR: Embryogenic callus and embryoid production was greater in embryos cultured scutellum up, and more embryoids were formed in the dark than in the light, while regenerated plants were grown to maturity in soil and had the normal diploid chromosome number of 14.
Journal ArticleDOI
Embryoid and plantlet formation from leaf segments of Dactylis glomerata L.
G. E. Hanning,B. V. Conger +1 more
TL;DR: The response for both callus and plantlet formation decreased with increasing distance both spatially and temporally from the shoot apex, providing further support for compentency (if not totipotency) of Gramineae leaf cells.
Journal ArticleDOI
Callus induction and plant regeneration from immature embryos of rye and triticale
Susan Eapen,P. S. Rao +1 more
TL;DR: Callus cultures were established from the scutellum, scutellar node and radicle region of immature embryos of rye and octoploid triticale on modified Murashige-Skoog basal medium supplemented with various growth regulators.
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