Showing papers in "Plant Science in 1990"

TL;DR: To eliminate the endogenous GUS activity, the effect of some water-soluble organic solvents on decreasing it was studied and it was found that addition of methanol at 20% volume to a GUS reaction mixture was very effective, lowering the endogenous activity to 0–15% of the origin in all plant extracts tested.

TL;DR: In this paper, the authors discuss the origins, causes and uses of Variation in Plant Tissue Cultures and their application in the control of fertility and embryo development in the field of plant cell and tissue culture.

TL;DR: In a genetical approach two single isoforms derived from a class I resp.

TL;DR: Membrane leakage caused by oxidant generating herbicides can also be used to predict drought tolerance; a much harder character to ascertain.

TL;DR: Chlamydomonas reinhardtii was shown to accumulate large amounts of starch following nutrient starvation under phototrophic, mixotrophic and heterotrophic conditions and this accumulation was clearly independent of plastome gene expression but depended on de novo cytoplasmic protein synthesis.

TL;DR: Phytochelatin formation was stimulated most effectively by Cd, less by Zn and Cu and negligibly by Ni, but with excess Cu the roots contained 45% more total glutathione than did the controls.

TL;DR: It seemed that the albino plant frequency was related to the genotypes rather than the medium composition, and the factors affecting pollen plant yield are discussed.

TL;DR: The transfer of kanamycin resistance of bacterial origin into wheat via pipetting Agrobacterium suspension into wheat spikelets via pollen-mediated gene transfer represents an alternative to protoplast-based transformation and leaf disk techniques by avoiding regeneration problems and somaclonal variation, introducing fertile plants.

TL;DR: In developing rapeseed the properties of the acyltransferases involved in de novo biosynthesis of triacylglycerol were compared especially with regard to their ability to utilize erucoyl-CoA as acyl donor, and it was revealed that erucic acid can enter position 1 of Triacyl glycerol.

TL;DR: Citrus protoplast-derived embryogenic calli can readily be induced to differentiate embryos which further develop into mature fruit bearing trees, resulting in transgenic plants.

TL;DR: The stage of zygotic embryo development for optimal somatic embryogenesis was found to be the early stage of polyembryony and the appearance and nutritional requirements of the established protoplast-derived cell suspension were similar to those of the original cell suspension.

TL;DR: A model for regulation of nodule formation in soybean is proposed, in which cortical cell division foci induce negative regulatory responses in the shoot through systemic signaling that ultimately results in blockage of further nodule development in the roots.

TL;DR: It is suggested that AgNO3 stimulates carrot somatic embryogenesis by inhibiting ethylene action; its implication in the control of ADC activity is discussed.

TL;DR: Two disarmed Ti-binary vectors in Agrobacterium tumefaciens have been used to produce viable transgenic strawberry plants and there was complete correlation between the presence of nopaline synthase activity and the ability of leaf segments to produce callus on a medium containing kanamycin.

TL;DR: All the molecular species of phosphatidylglycerol (PG) containing trans -hexadecenoic acid (16:1t) were severely reduced by drought stress, this reduction being related to a parallel increase in the 16:0-containing molecular species.

TL;DR: Cucumber seeds thus contain a highly basic endochitinase/lysozyme which disappears almost completely 3 days after germination, and the role of this seed hydrolase has yet to be determined.

TL;DR: Embryogenic callus further developed somatic embryos with distinct scutellar- and coleoptile-like structures, which produced normal, fertile plants with no apparent morphological abnormalities.

TL;DR: RNA blots with a petunia EPSPS cDNA demonstrate a correlation between levels of tolerance and steady state level of EPSPS mRNA, indicating that selection has resulted in a stable genetic modification.

TL;DR: Results show that under water stress the tripartite symbiosis maintains higher values of photosynthetic and nodule activities than non-myocorrhizal plants suggesting enhanced drought tolerance by mycorrhIZal condition.

TL;DR: Embryogenic calli of black spruce were initiated in vitro from immature zygotic embryos collected during the maturation period, and with mature zygosis dissected from seed which had been stored for 13 years.

TL;DR: Two distinct types of somatic embryos were induced from the cotyledonary region of callused zygotic embryos of Feijoa sellowiana cultured in MS medium supplemented with a wide range of 2,4-dichlorophenoxyacetic acid concentrations to induce somatic embryogenesis.

TL;DR: It is postulated that ring-methyl hydroxylase of wheat belongs to the family of cytochrome P -450 monooxygenases, which could also be formed in the presence of cumene hydroperoxide, and the N -demethylase activity was not affected by CO.

TL;DR: The results highlight the importance of PPi as an autonomous energy donor in the cytosol of higher plants.

TL;DR: Correlation of resistance to the different viruses was observed in aneuploid hybrids lacking parental chromosomes, suggesting that resistance/susceptibility is associated with the loss of individual S. brevidens or S. tuberosum chromosomes.

TL;DR: With longer incubation on MS medium the shoot-forming capacity of the explants declined, and after 8 days it was completely lost, and the possible usefulness of B. juncea cotyledons as an experimental system for detailed studies of morphogenesis and genetic transformation is discussed.

TL;DR: It is demonstrated that isoprene biosynthesis and emission are independent of photorespiration.

TL;DR: Kinetic analysis of the partially purified preparation revealed there are small changes in the affinity for the substrates, fructose 6-phosphate and UDP-glucose, and largeChanges in the sensitivity to the allosteric activator Glc6P, and inhibitor, Pi.

TL;DR: There was a positive correlation of increased conversion frequency and high embryo fresh/dry weights, dry matter content and starch levels when the somatic embryos were matured on ABA medium.

TL;DR: The regeneration protocol studied using immature leaf explants taken from in vitro shoots and greenhouse grown plants of the strawberry cv.

TL;DR: Neither nopaline nor octopine was ever detected in extracts of tumour-free inoculation sites which had been inoculated with virulent or avirulent strains, suggesting that transformation has occurred.