Journal ArticleDOI
Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability
TLDR
An efficient procedure for obtaining somatic hybrids between B. oleracea and B. campestris has been developed, showing the occurrence of multiple fusion and chromosome loss during the culture.Abstract:
An efficient procedure for obtaining somatic hybrids between B. oleracea and B. campestris has been developed. Hypocotyl protoplasts of B. oleracea were fused with mesophyll protoplasts from three different varieties of B. campestris by the polyethylene glycoldimethylsulfoxide method. The selection of somatic hybrids utilized the inactivation of B. oleracea protoplasts by iodoacetamide (IOA) and the low regeneration ability of B. campestris. The efficiency of recovery of somatic hybrids depended upon the IOA concentration, and when 15 mM IOA was used, 90% of the regenerated plants were found to be hybrid. The somatic hybrids were examined for i) leaf morphology, ii) leucine aminopeptidase (LAP) isozyme and iii) chromosome number. All the hybrids had intermediate leaf morphology and possessed LAP isozymes of both parental species. The chromosome analysis revealed a considerable variation in chromosome number of somatic hybrids, showing the occurrence of multiple fusion and chromosome loss during the culture. Some of the hybrids flowered and set seeds.read more
Citations
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Journal Article
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoïd protein electrophoresis.
Book ChapterDOI
Brassica and Its Close Allies: Cytogenetics and Evolution
Journal ArticleDOI
Resynthesis of Brassies napus L. through Interspecific Hybridization between B. alboglabra Bailey and B. campestris L. with Special Emphasis on Seed Colour
TL;DR: Brassica napus L. was resynthesized through interspecific hybridization between B. alboglabra Bailey and B. campestris L. with the objective or developing yellow-seeded forms of this species and the use of the embryo rescue culture technique greatly increased the number of surviving hybrid embryos.
Journal ArticleDOI
Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing)
TL;DR: This study clearly demonstrates the totipotency of protoplasts in graminaceous monocots in rice and successfully hybridizes barnyard grass and rice sativa L.
Book ChapterDOI
The potential of somatic hybridization in crop breeding
Sylvia Waara,Kristina Glimelius +1 more
TL;DR: In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed and it is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop.
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Haploid Plants from Pollen Grains
J. P. Nitsch,C. Nitsch +1 more
TL;DR: A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains, structures which develop in stages similar to those of zygotic embryos.
Journal ArticleDOI
Nutritional Requirements for Growth of Vicia hajastana Cells and Protoplasts at a Very Low Population Density in Liquid Media
K. N. Kao,M. R. Michayluk +1 more
TL;DR: Vicia cells were able to grow in a mineral-salt solution supplemented with sucrose (or glucose), a few vitamins, and 2,4-dichlorophenoxyacetic acid but were not able to survive when cultured at a low population density unless the medium was supplemented with zeatin, naphthalene-1-acetic Acid, nucleic-acid bases, amino acids, other sugars, sugar alcohols, and organic acids.
Journal ArticleDOI
Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion
TL;DR: Rapeseed plants have been regenerated after fusion between protoplasts bearing cytoplasms of different genera and transfer of chloroplasts has been confirmed by restriction cp DNA analysis and two dimensional thylakoid protein electrophoresis.
Journal ArticleDOI
Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion
TL;DR: It is reported here, however, that the mitochondrial (mt) DNAs of cybrids are different from those of the parents and from the mixture of the two.
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