Journal ArticleDOI
Spin-label study of segmental flexibility of anti-hapten antibodies. Precipitating pig anti-Dnp antibody is more flexible than non-precipitating.
TLDR
This study determined the 7 values of the early and late pig anti-Dnp antibodies with precipitating and non-precipitating properties and found the rotational correlation times i of immunoglobulin molecules and their fragments to be in fair agreement with the results of fluorescence polarization experiments.About:
This article is published in FEBS Letters.The article was published on 1979-05-01. It has received 21 citations till now. The article focuses on the topics: Hapten.read more
Citations
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Journal ArticleDOI
The study on the interaction between human serum albumin and a new reagent with antitumour activity by spectrophotometric methods
TL;DR: Experimental results and theoretical data clarified that HNF could bind to HSA and be effectively transported and eliminated in body, which could be a useful guideline for further drug design.
Journal ArticleDOI
The Neonatal Fc Receptor (FcRn): A Misnomer?
Michal Pyzik,Kine Marita Knudsen Sand,Kine Marita Knudsen Sand,Jonathan J. Hubbard,Jonathan J. Hubbard,Jan Terje Andersen,Inger Sandlie,Richard S. Blumberg,Richard S. Blumberg +8 more
TL;DR: The Neonatal Fc receptor (FcRn) as discussed by the authors is the most common type of antibody found in circulation and extracellular fluids and it has been recognized as an immune receptor by interacting with and facilitating antigen presentation of peptides derived from IgG immune complexes.
Book ChapterDOI
Internal movements in immunoglobulin molecules.
TL;DR: This chapter summarizes the various studies of the movement of Ig subunits—that is, segmental flexibility as well as internal motion in the subunits themselves, and discusses the functional implications of these motions.
Journal ArticleDOI
Temperature and pH dependence of immunoglobulin G conformation
TL;DR: The assumption is that the main conformational change observed appears either in the hinge region of the molecular or in the interdomain areas separating the constant and the variable domains of the Fab parts.
Journal ArticleDOI
Segmental flexibility in pig immunoglobulin G studied by neutron spin-echo technique.
TL;DR: The dynamic behavior of pig immunoglobulin G in deuterium oxide solutions was investigated by the neutron spin‐echo technique, which makes it possible to study intramolecular motion without introducing probes into the macromolecule.
References
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Journal ArticleDOI
Rotational correlation time of spin-labeled α-chymotrypsin
TL;DR: In this article, the positions of the high field hyperfine components in the paramagnetic resonance spectra of spin-labeled enzymes have been measured as a function of solvent viscosity and have been used to determine a rotational correlation time of 12 nanoseconds for α-chymotrypsin in water at 20° C.
Journal ArticleDOI
Conversion of incomplete antibodies to direct agglutinins by mild reduction: evidence for segmental flexibility within the Fc fragment of immunoglobulin G.
TL;DR: Results suggest that mild reduction of the antibody imparts sufficient freedom to permit bridging between cells and are interpreted as evidence that the interheavy-chain disulfide bonds restrict segmental flexibility within the Fc fragment of IgG.
Journal ArticleDOI
Rotational correlation times of IgG and its fragments spin-labeled at carbohydrate or protein moieties. Spatially fixed position of the Fc carbohydrate.
TL;DR: The r values of IgG and its Fc fragment spin-labeled at carbohydrates are determined by means of the method, which permits evaluation of the rotation of spinqabel relative to a macromolecule, and provide direct evidence for the existence of an internal lability of Fc fragments and for the rigid fixation of its carbohydrate component in solution.
Journal ArticleDOI
Fluorescence polarization analysis of various immunoglobulins. Dependence of rotational relaxation time on protein concentration and on ability to precipitate with antigen.
TL;DR: It is shown that the rotational relaxation times of immunoglobulins exhibit a significant dependence on Immunoglobulin concentration, which is tentatively explained by loosening of the structure of the Fc part of the molecule in concentrations below 2 PM.
Journal ArticleDOI
Different types of precipitating antibodies in early and late porcine anti-dinitrophenyl sera.
TL;DR: It is suggested that the early and late antibodies represent closely related subclasses of porcine IgG differing by the shape and/or flexibility of the molecule.