Journal ArticleDOI
Steroid-protein interactions. XXII. Extrapolation technique for determination of corticosteroid-binding globulin by gel filtration.
Walter Hoffmann,Ulrich Westphal +1 more
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A method has been developed for the quantification of the corticosteroid-binding globulin (CBG) which eliminates the disadvantages of the gel filtration technique (dissociation, possible reversible adsorption).About:
This article is published in Analytical Biochemistry.The article was published on 1969-10-01. It has received 17 citations till now. The article focuses on the topics: Sephadex.read more
Citations
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Recent advances in chromatographic and electrophoretic methods for the study of drug-protein interactions
David S. Hage,Stacey A. Tweed +1 more
TL;DR: This review examines various chromatographic and electrophoretic methods that have been developed to study drug-protein interactions and demonstrates how the same methods, particularly when used in high-performance liquid chromatography or capillary electrophoresis systems, can be employed as rapid screening tools for investigating the binding of different forms of a chiral drug to a protein.
Journal ArticleDOI
Metabolism of the Vitamin A Transporting Protein Complex
TL;DR: It is proposed that most RBP is catabolized from the pool of the uneomplexed species and as much as one third of the plasma vitamin A may be deposited in the kidney tubuli on degradation of RBP.
Journal ArticleDOI
Chromatographic and electrophoretic studies of protein binding to chiral solutes.
TL;DR: This review examines data that have been obtained on protein interactions by various chromatographic and electrophoretic methods, especially those based on either high-performance liquid chromatography or capillary electrophoresis.
Journal ArticleDOI
Biochemical and clinical observations in a pregnancy with placental sulphatase and other enzyme deficiencies
TL;DR: The oestrogen excretion of a pregnant woman, subsequently shown to have placental sulphatase deficiency, was only 1‐2 mg/24 hr, whereas pregnanediol and 17‐oxogenic steroid excretion were within the normal ranges, whereas Plasma concentrations of oestradiol‐17β, progesterone, 17α‐hydroxyprogesterone, 11β‐hydrophic‐steroids and corticosteroid‐binding globulin were subnormal.
Journal ArticleDOI
Improved gel-filtration method for analysis of estrogen receptor binding
TL;DR: The dissociation constant of the estrogen receptor complex was determined with greater accuracy than previously determined by submitting the corrected receptor binding data to Scatchard plot analysis.
References
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Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
Journal ArticleDOI
A graphic method for the determination and presentation of binding parameters in a complex system
TL;DR: A graphic method is presented which correlates on a single graph the physicochemical parameters of a binding system consisting of one ligand and one or more binding macromolecules each having one ormore groups of mutually noninteracting binding sites.
Journal ArticleDOI
Evaluation of Silber-Porter Procedure for Determination of Plasma Hydrocortisone
Journal ArticleDOI
Steroid-protein interaction with particular reference to testosterone binding by human serum.
William H. Pearlman,Odette Crépy +1 more
TL;DR: A sensitive and rapid method for the detection and quantitative estimation of serum protein, or proteins, exhibiting a specific or high binding affinity for testosterone may also prove to be useful in the general study of steroid-protein interaction.
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Measurement of Corticosteroid-Binding Globulin in Man
TL;DR: A method for quantitation of corticosteroid-binding globulin (CBG) has been developed utilizing gel filtration and 4-14C cortisol and increase in binding with decrease in assay temperature was again demonstrated for normal and pregnancy serum utilizing the present method.
Related Papers (5)
Steroid-protein interactions. XVI. Isolation and characterization of the corticosteroid-binding globulin of the rabbit.
Gerald J. Chader,Ulrich Westphal +1 more