Determination of serum proteins by means of the biuret reaction.
TLDR
An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.About:
This article is published in Journal of Biological Chemistry.The article was published on 1949-02-01 and is currently open access. It has received 15717 citations till now. The article focuses on the topics: Biuret test & Reagent.read more
Citations
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Journal ArticleDOI
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum
TL;DR: The relatively low density of the lipoproteins was utilized by Lindgren, Elliott, and Gofman to separate them from the other serum proteins by ultracentrifugal flotation, and quantitation was subsequently performed by refractometric methods in the analytical ultracentRifuge.
Book ChapterDOI
Spectrophotometric and turbidimetric methods for measuring proteins
TL;DR: The turbidity produced when protein is mixed with low concentrations of any of the common protein precipitants can be used as an index of protein concentration, and this advantage is used to eliminate the interference of nucleic acids in the estimation of protein.
References
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An accurate and rapid method for the determination of proteins in small amounts of blood serum and plasma.
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The biuret reaction in the determination of serum proteins i. a study of the conditions necessary for the production of a stable color which bears a quantitative relationship to the protein concentration
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Micro-Kjeldahl Determination of Nitrogen.A New Indicator and An Improved Rapid Method
T. S. Ma,G. Zuazaga +1 more
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