Journal ArticleDOI
Stimulation of corticosteroid biosynthesis by angiotensin I [des-asp1]angiotensin I, angiotensin II and [des-asp1]-angiotensin II in bovine adrenal fasciculata cells.
TLDR
Data demonstrate a potent steroidogenic activity for AII as well as AI, [des-asp1]AI and [ Des-asp2-arg2]AII in bovine adrenal fasciculata cells and a common receptor site for all four peptides is suggested.Abstract:
The effect of angiotensin I (AI), angiotensin II (All), [des-asp1]AI, [des-asp1]AII and [des-asp1-arg2]AII on corticosteroid production in isolated fasciculata cells from bovine adrenals has been studied. All and [des-asp1]AII in concentrations ranging from 10−9M to 10−6M had a potent stimulatory effect on steroid biosynthesis. The dose-response curves for both peptides were identical. AI was about 3 times less potent than All and [des-asp1]AII. The effect of AI was not due to its conversion to All. [des-asp1]AI was as active as AI. No significant conversion to [des-asp1]AII was observed. [des-asp1-arg2]AII had only a minimal effect on steroidogenesis. The structural analog [sar1,-ala8]AII inhibited all angiotensins specifically and competitively. The affinity of the cellular binding site was higher for All and [des-asp1]AII than for [sar1ala8]AII, but lower for AI and [des-asp1]AI than for the inhibitor. Combination of submaximal doses of AI and All resulted in an additive effect on steroid production. B...read more
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Journal ArticleDOI
Cellular Biology of the Renin-Angiotensin Systems
TL;DR: Evidence suggests that renin can be synthesized at local tissue sites and that these local renin-angiotensin systems subserve important physiologic functions.
Journal ArticleDOI
Regulation of angiotensin II receptors and steroidogenic responsiveness in cultured bovine fasciculata and glomerulosa adrenal cells
TL;DR: In contrast to the results reported in vivo in the rat, where A-II leads to up-regulation of its own receptors on glomerulosa cells and increases steroidogenic responsiveness, this peptide results in both down-regulation and desensitization in cultured bovine fasciculata and glomerULosa cells.
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Localization of aldosterone and corticosterone in the central nervous system, assessed by quantitative autoradiography
TL;DR: A receptor readily shared by aldosterone, corticosterone, 18-OH-DOC and DOC, but not by dihydrotestosterone, is widely distributed throughout the CNS and may be present in hippocampal areas CA1 and CA2, which means that both these as well as the receptor accepting dihydotestosterone can be located within the same cell.
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The adrenal cortex.
TL;DR: Examination of the action of adrenocorticotropin on specific molecular events within adrenal cells may result in better understanding of polypeptide hormone action in general.