Reference EntryDOI
Strategies for the Analysis of Plant Lipids
Irina A. Guschina,John L. Harwood +1 more
- pp 1-17
TLDR
This chapter describes the most useful methods currently in use including the use of isotopes to study metabolism, as well as prospects for useful future developments.Abstract:
Lipids are vital constituents of cells – as membrane components, energy stores, signaling molecules, and surface compounds. In this article, we describe major lipids, how they can be extracted, and precautions in their handling. Specific methods for lipid analysis including radioisotope work, thin‐layer and gas–liquid chromatography and mass spectroscopy are then described and discussed.read more
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Lipids, fatty acids and pigments of leaves dictamnus caucasicus fisch. ex grossh. (ru-taceae)
E. S. Bogdanova,V. N. Nesterov,Stepan Aleksandrovich Senator,Vladimir Mikhaylovich Vasjukov,Olga A. Rozentsvet +4 more
TL;DR: In this paper, the qualitative and quantitative composition of components lipids, fatty acids, and pigments isolated from the leaves of Dictamnus caucasicus Fisch was presented for the first time.
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Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Lipids and lipid metabolism in eukaryotic algae
TL;DR: Attempts to discover genes that code for expression of the various proteins involved in the production of very long-chain polyunsaturated fatty acids such as arachidonic, eicosapentaenoic and docosahexaenoic acids are described.
Journal ArticleDOI
Profiling Membrane Lipids in Plant Stress Responses ROLE OF PHOSPHOLIPASE Dα IN FREEZING-INDUCED LIPID CHANGES IN ARABIDOPSIS
Ruth Welti,Weiqi Li,Maoyin Li,Yongming Sang,Homigol Biesiada,Han-E Zhou,C.B. Rajashekar,Todd D. Williams,Xuemin Wang +8 more
TL;DR: Data suggest that PC, rather than PE and PG, is the majorin vivo substrate of PLDα, and the greater loss of PC and increase in PA in wild-type plants as compared with PLD α-deficient plants may be responsible for destabilizing membrane bilayer structure.
Journal ArticleDOI
Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry.
TL;DR: The optimized ionization and fragmentation conditions described together with the principle of internal standardization by nonnatural analogues allow the rapid and quantitative determination of membrane lipid compositions down to sample amounts of 1000 cells.