Structure of the piriform cortex of the opossum. II. Fine structure of cell bodies and neuropil.

TLDR: A close similarity in ultrastructure of many features has been demonstrated between this pylogenetically old cortex in a primitive mammal and the neocortex of higher mammals.

Abstract: The fine structure of cell bodies and neuropil in the piriform cortex of the opossum has been examined. A close similarity in ultrastructure of many features has been demonstrated between this pylogenetically old cortex in a primitive mammal and the neocortex of higher mammals. Cell bodies of pyramidal cells are very similar to those in the neocortex: The nucleus is pale with a smooth surface, the cytoplasm has a modest number of organelles, and the soma receives a small number of exclusively symmetrical synapses. Semilunar cells, which have apical but no basal den-drites, are very similar to pyramidal cells in ultrastructure of their cell bodies. Two populations of neurons with nonpyramidal ultrastructural features have been distinguished: (1) cells in layer III that closely resemble the well-known large multipolar cells in neocortex by virtue of a large number of symmetrical and asymmetrical somatic synapses and long cisterns of rough endoplasmic reticulum (ER); and (2) large cells in layer I with very few somatic synapses, a large number of mitochondria, and short cisterns of rough ER that may correspond to cells with somatic appendages described with the Golgi method. Large numbers of profiles are found in all layers that contain round vesicles and make asymmetrical synapses onto dendritic spines, and occasionally, dendritic shafts. Theseprofileshavedistinctly different morphological features in layer Ia, in which olfactory bulb afferents are concentrated, and in layers Ib, II, and III, which contain terminals of association and commis-sural fibers. A smaller number of profiles containing pleomorphic vesicles make symmetrical contacts onto initial segments, dendritic shafts, cell bodies, and occasionally, dendritic spines. Most dendritic spines in all layers are small to medium in size (0.3–1.2 μm) and presumably originate from pyramidal cells. In layer Ia, however, large, flattened spines are also present which appear to originate from semi-lunar cells. In layer III, and to a lesser extent other layers, large irregular spines are present that may be branched appendages on dendrites of complex appendage cells (Haberly, 1983).