Journal ArticleDOI
Studies on tRNA nucleotidyltransferase from baker's yeast. 1. Purification of the enzyme. Protection against thermal inactivation and inhibition by several substrates.
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This article is published in FEBS Journal.The article was published on 1974-12-16. It has received 70 citations till now. The article focuses on the topics: tRNA nucleotidyltransferase & Saccharomyces cerevisiae.read more
Citations
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Probing the structure of RNAs in solution
Chantal Ehresmann,Florence Baudin,Marylène Mougel,Pascale Romby,Jean-Pierre Ebel,Bernard Ehresmann +5 more
TL;DR: In this paper, the various structure probes used so far are described, and their utilization is discussed.
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The role of exportin-t in selective nuclear export of mature tRNAs.
TL;DR: Results suggest that at least two mechanisms are involved in discrimination of pre‐tRNAs and mature tRNAs prior to nuclear export, including tRNA precursors.
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Yeast tRNAAsp tertiary structure in solution and areas of interaction of the tRNA with aspartyl-tRNA synthetase. A comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea.
Pascale Romby,Dino Moras,Marc Bergdoll,Philippe Dumas,Valentin V. Vlassov,Eric Westhof,Jean Pierre Ebel,Richard Giegé +7 more
TL;DR: The existence of different interaction schemes between tRNAs and aminoacyl-tRNA synthetases, correlated with the oligomeric structure of the enzyme, is proposed.
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Tertiary Structure of tRNAs in Solution Monitored by Phosphodiester Modification with Ethylnitrosourea
TL;DR: A comparison of the patterns of phosphate alkylation in several tRNAs, essentially in their T psi C arms, revealed a striking similarity, suggesting that the folding of these t RNAs is essentially similar, as shown by others.
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Cleavage of tRNA with imidazole and spermine imidazole constructs: a new approach for probing RNA structure.
TL;DR: Cleavages in a viral RNA fragment recapitulating a tRNA-like domain were found in agreement with the model of this molecule that accounts for its functional properties, thus illustrating the potential of the imidazole-derived reagents as structural probes for solution mapping of RNAs.
References
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Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method
Rusty J. Mans,G.David Novelli +1 more
TL;DR: A rapid and sensitive method has been described for the measurement of amino acid incorporation in cell-free systems and has been applied to both C14- and H3-labeled proteins.
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The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations
TL;DR: A nomenclature to facilitate discussion of possible mechanisms for enzyme-catalyzed reactions with more than one substrate or product is proposed and examples are given to illustrate the types of information about reaction mechanisms that can be obtained.
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The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory.
TL;DR: A nomenclature is proposed to describe different types of inhibitions of enzyme-catalyzed reactions, particularly for reactions with more than one substrate and product, and the rate equations for such inhibitions are discussed.
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Fluorescence studies of nucleotides and polynucleotides. I. Formycin, 2-aminopurine riboside, 2,6-diaminopurine riboside, and their derivatives.
TL;DR: Some potential applications of these analogues in studies of the structure and function of nucleic acids and proteins are: analysis of protein-nucleic acid interaction, polynucleotide conformation and conformational transitions, enzyme-coenzyme interaction, and nuclease assay.
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The kinetics of enzyme-catalyzed reactions with two or more substrates or products: III. Prediction of initial velocity and inhibition patterns by inspection
TL;DR: This method is applicable to any non-random mechanism without alternate reaction sequences and it is shown that initial velocity and dead end and product inhibition patterns by inspection of the mechanism are similar.