Journal ArticleDOI
Substrate-binding properties of firefly luciferase. II. ATP-binding site.
TLDR
Kinetic as well as inhibition studies showed that uncomplexed ATP is also bound to the luciferase, and is a competitive inhibitor with respect to MgATP, and many divalent metal ions can replace Mg 2+ in the light reaction.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-11-01. It has received 97 citations till now. The article focuses on the topics: Luciferase & Luciferin.read more
Citations
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Journal ArticleDOI
Analytical information obtainable by evaluation of the time course of firefly bioluminescence in the assay of ATP.
Arne Lundin,Anders Thore +1 more
TL;DR: The analytical accuracy of the firefly assay of ATP has been studied and is discussed with special reference to interference with the time course of the bioluminescence, with results shown to result in erroneous assay in a bacterial extract.
Journal ArticleDOI
The chemical mechanism and evolutionary development of beetle bioluminescence
TL;DR: Bioluminescence, as a phenotype, has many evolutionary origins, and thus is an example of natural reinvention many times over, although peculiar, it arises from the same biochemical principles and evolutionary mechanisms as other biochemical reactions.
Journal ArticleDOI
Firefly luminescence: A historical perspective and recent developments
TL;DR: Significant advances have occurred regarding the authors' knowledge of firefly luciferase mechanisms, although most of this progress was an outcome of molecular biology and structural studies, and is presented in a historical perspective.
Journal ArticleDOI
Kinetic analysis and modeling of Firefly luciferase as a quantitative reporter gene in live mammalian cells
TL;DR: A dynamic analysis of luciferase activity in the context of living mammalian cells is reported, which determined the relative light units measured in living cells to be proportional to that found in cell lysate and modeled luciferases activity in live cells for the purposes of understanding and translating the Luciferase signal into a more effective metric of gene expression and cell behavior.
Journal ArticleDOI
Oxidation and Reduction of Iron by Acidophilic Bacteria
Jack T. Pronk,D. Barrie Johnson +1 more
TL;DR: Recent new developments in the field of acidophilic iron metabolism are reviewed and new heterotrophic isolates have been described that are capable of oxidizing ferrous iron.
References
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Journal ArticleDOI
The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
Order of Stability of Metal Complexes
H. Irving,R. J. P. Williams +1 more
TL;DR: In this article, the stability of complexes of bivalent metal ions follows the order of the order in which they are formed, and it is shown that the stability is not correlated with the order.
Journal ArticleDOI
Interaction of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity
Journal ArticleDOI
Nuclear magnetic resonance spectra of adenosine di- and triphosphate. II. Effect of complexing with divalent metal ions.
TL;DR: The chemical shifts of the proton magnetic resonance spectra in the nucleotides could be compared with the shifts in the metal complexes to determine the interaction of the metal ion with the adenine ring, thus specifying the nature of the met.al complexes formed.
Related Papers (5)
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