Journal ArticleDOI
Surface effects in preparation of cell-size liposomes
TLDR
This paper showed that pyrex glass beads can increase the surface area of lipid-glass contact by more than 100 times in the presence of polyethylene glass beads, which is more than 5 times larger than in the control experiments without glass beads.About:
This article is published in FEBS Letters.The article was published on 1984-10-29. It has received 19 citations till now. The article focuses on the topics: Lamellar phase & Liposome.read more
Citations
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Book ChapterDOI
Electrical breakdown, electropermeabilization and electrofusion
TL;DR: The techniques for electrical breakdown, electropermeabilization and electrofusion could be an important tool in this process, since the high electrical fields occurring naturally in the membrane play an important role in the selective transport of substances across the membrane as well as in natural regulatory processes.
Book ChapterDOI
A mechanism of liposome electroformation
TL;DR: In this article, the authors present new data and theoretical estimates which allow them to suggest a possible mechanism of liposome electroformation, which can include at least three basic stages: (1) separation of interacting membranes, (2) instability of bending which can result from negative membrane tension due to surface and line tension and (3) the bending itself and closing of the membranes.
Journal ArticleDOI
Lipid swelling and liposome formation mediated by electric fields
TL;DR: In this article, the authors discuss theoretical and experimental results of the effects of dc fields and present new data and estimates for lipid swelling and liposome formation on metal electrodes and glass surfaces in ac fields and for swelling of positively charged lipid mixtures in dc fields.
Journal ArticleDOI
Giant Vesicles as Biochemical Compartments: The Use of Microinjection Techniques
TL;DR: Detailed experimental procedures are described for the preparation of thin-walled giant phosphatidylcholine vesicles, which are useful for microinjection experiments and how the experimental parameters influence the vesicle formation process is investigated.
Book ChapterDOI
Lipid swelling and liposome formation on solid surfaces in external electric fields
D. S. Dimitrov,M. I. Angelova +1 more
TL;DR: In this paper, the authors present experimental results and theoretical estimates for these effects and present new data and estimates for the effects of lipid charge and frequency of the electric field on liposome formation.
References
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Journal ArticleDOI
Comparative Properties and Methods of Preparation of Lipid Vesicles (Liposomes)
TL;DR: This research attacked the mode of action of phosphatidylcholine-like deposits in response to the presence of ribonucleic acid by exploiting its role as a “spatially aggregating substance” in the response to EMT.
Journal ArticleDOI
Formation and properties of cell-size lipid bilayer vesicles.
P. Mueller,T.F. Chien,B. Rudy +2 more
TL;DR: G gramicidin increases the membrane conductance and monazomycin induces voltage-dependent gating thus providing further evidence that the vesicles are bounded by a single bilayer.
Journal ArticleDOI
Dynamic interactions between approaching surfaces of biological interest
TL;DR: In this article, the basic physical laws describing the dynamic behavior of interfaces and the liquid film between them are presented and the thin film approximation is used in order to simplify them, and the interactions between surface shape perturbations which are important for liquid film stability are considered with special attention to the case of membranes.
Journal ArticleDOI
Preparation of large unilamellar vesicles
TL;DR: A simple procedure for obtaining a high yield of single bilayer vesicle, almost uncontaminated by multilamellar vesicles is described, based on the use of thin lipid films and relatively high temperature.
Journal ArticleDOI
Stability of multilayered thin liquid films and applications to membrane systems
TL;DR: In this paper, a general dispersion equation which describes the linear, hydrodynamic stability of a system of arbitrary number of interacting plane-parallel, isotropic, viscous films with tangentially immobile interfaces in the longwavelength limit is obtained and analyzed in detail for the particular cases of one, two, and three films.