Journal ArticleDOI
Synthesis of 5'-oligonucleotide hydrazide derivatives and their use in preparation of enzyme-nucleic acid hybridization probes.
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TLDR
A hydrazone-based method for conjugating synthetic nucleic acids and reporter molecules for use as nonradioactive hybridization probes is presented and was able to detect 7 amol of target DNA in 1 h, using a colorimetric assay.About:
This article is published in Analytical Biochemistry.The article was published on 1989-04-01. It has received 46 citations till now. The article focuses on the topics: Oligonucleotide & Hydrazide.read more
Citations
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Conjugates of oligonucleotides and modified oligonucleotides: a review of their synthesis and properties
Journal ArticleDOI
Applications of dioxetane chemiluminescent probes to molecular biology
Stephan Beck,Hubert Köster +1 more
TL;DR: One of the most promising recent developments presents a method based on enzymatically triggered chemiluminescence that is compared to conventional methods and applications in molecular biology and immunology.
Journal ArticleDOI
Use of maleimide-thiol coupling chemistry for efficient syntheses of oligonucleotide-enzyme conjugate hybridization probes.
TL;DR: Two general methods which exploit the reactivity of sulfhydryl groups toward maleimides are described for the synthesis of oligonucleotide-enzyme conjugates for use as nonradioisotopic hybridization probes.
Patent
End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
Soumitra S. Ghosh,Eoin Fahy +1 more
TL;DR: In this article, a method for covalent attachment of oligonucleotides to solid supports such that substantially all of the oligonuclotides are attached via their 5'-ends is provided.
Patent
DNA-protein fusions and uses thereof
TL;DR: In this paper, the authors proposed methods for covalently tagging proteins with their encoding DNA sequences as shown in the Figure 1, and these DNA-protein fusions may be used in molecular evolution and recognition techniques.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Peroxidase-labeled antibody. A new method of conjugation
Paul K. Nakane,Akira Kawaoi +1 more
TL;DR: A new method of conjugating horseradish peroxidase with proteins was developed by oxidizing the carbohydrate moiety with sodium periodate and bound to free amino groups of proteins unidirectionally at high efficiencies.
Journal ArticleDOI
Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin
TL;DR: The sensitivity of detection of target RNA in dot-blots and Northern blots was equivalent to that obtained with 32p-labelled DNA probes, and photobiotin was also used for the labelling of proteins with biotin.
Journal ArticleDOI
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use hybridization probes
TL;DR: Short synthetic oligonucleotides have been covalently cross-linked to alkaline phosphatase using the homobifunctional reagent disuccinimidyl suberate to produce oligomer-enzyme conjugates that can hybridize to target DNA fixed to nitrocellulose within 15 minutes.
Journal ArticleDOI
High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris
James M. Cregg,Juerg Friedrich Tschopp,Cathy A. Stillman,Robert Steven Siegel,M. Akong,William Scot Craig,Richard Gordon Buckholz,Knut Madden,P. A. Kellaris,Geneva Ruth Davis,B. L. Smiley,J. Cruze,R. Torregrossa,G. Velic ecedil,lebi,Gregory Patrick Thill +15 more
TL;DR: The methylotrophic industrial yeast, Pi–chia pastoris, has been developed as a host system for the large–scale production of heterologous proteins, and the synthesis of hepatitis B surface antigen (HBsAg) is described.