Synthesis of Cellulase during Abscission of Phaseolus vulgaris Leaf Explants
Lowell N. Lewis,Joseph E. Varner +1 more
Reads0
Chats0
TLDR
When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in (2)H(2)O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed, indicating that the increase in cellulase activity during abscissions is a result of the synthesis of new protein.Abstract:
When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in (2)H(2)O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed. These data indicate that the increase in cellulase activity during abscission is a result of the synthesis of new protein. Two differentially soluble forms of cellulase are present in the abscission zone. The form which is soluble only in a high salt buffer seems more closely related to the abscission process than the form which is soluble in dilute buffer. The correlation between changes in pull force and increase in cellulase activity and the effects of several hormones on cellulase activity are discussed.read more
Citations
More filters
Journal Article
Interactive effects of coating method and storage period on quality of carrot (cv. nantes) during ambient storage
TL;DR: In this paper, the interactive effects of Coating Methods (CM) and Storage Periods (SP) on Nantes carrot during ambient storage at temperature of 25°C and 65% relative humidity were investigated for some qualitative characteristics including water content, total soluble solids (TSS), reducing sugar and firmness.
Book ChapterDOI
β-Glucanases in Higher Plants: Localization, Potential Functions, and Regulation
TL;DR: The major β-linked glucan polysaccharides are β1,4-glucan (cellulose) and β 1,3-oglucans (e.g., curdlan, callose).
Journal ArticleDOI
Effect of Ethylene on Plasma Membrane Density in Kidney Bean Abscission Zones
Don E. Koehler,Lowell N. Lewis +1 more
TL;DR: An increase in the buoyant density of the plasma membrane fraction from 1.165 to 1.175 grams per cubic centimeter was observed, consistent with the loss of lipids from the senescent cells of the abscission zone.
Journal ArticleDOI
Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
TL;DR: It is concluded that the soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
Journal ArticleDOI
Abscission in the Tumbleweed Kochia indica: Ethylene, Cellulase, and Anatomical Structure
TL;DR: It is suggested that ethylene induces cellulase activity, probably through intermediate steps, which in turn dissolves the walls of the parenchyma cells, and in the transition region this causes abscission, while in the root this causes only softening.
References
More filters
Journal ArticleDOI
A photometric adaptation of the somogyi method for the determination of glucose
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.
Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
Philip Filner,Joseph E. Varner +1 more
Journal ArticleDOI
Senescence, Abscission and Cellulase Activity in Phaseolus vulgaris
TL;DR: Before abscissions, high cellulase activity develops in cells of the abscission zone, suggesting that this enzyme plays an important part in cell separation.