Journal ArticleDOI
Synthesis of ubiquinone and cholesterol in human fibroblasts: regulation of a branched pathway.
TLDR
The current data are compatible with a regulatory mechanism in which LDL inhibits cholesterol synthesis in fibroblasts at two points: at the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase, thereby inhibiting mevalonate synthesis, and at one or more points distal to the last intermediate common to the cholesterol and ubiquinone-10 biosynthetic pathways.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1979-01-01. It has received 135 citations till now. The article focuses on the topics: HMG-CoA reductase & Cholesterol.read more
Citations
More filters
Journal ArticleDOI
Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth
TL;DR: The availability of compactin (ML-236B), a potent competitive inhibitor of 3-hydroxy-3-methylglutaryl Coenzyme A reductase, has permitted the demonstration of a hitherto unsuspected aspect of mevalonate metabolism and isoprenoid synthesis in cultured mammalian cells.
Journal ArticleDOI
Relation of cholesterol and mevalonic acid to the cell cycle in smooth muscle and swiss 3T3 cells stimulated to divide by platelet-derived growth factor.
Journal ArticleDOI
The intracellular transport of low density lipoprotein-derived cholesterol is defective in Niemann-Pick type C fibroblasts.
TL;DR: The results indicate that a cholesterol transport defect exists in NPC that is specific for LDL-derived cholesterol.
Journal ArticleDOI
Isoprenoid biosynthesis in plants: carbon partitioning within the cytoplasmic pathway
TL;DR: In this article, isoprenoid biosynthesis in plants: Carbon Partitioning Within the Cytoplasmic Pathway, is discussed and discussed in the context of plant carbon partitioning.
Journal ArticleDOI
Effects of an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme a reductase on serum lipoproteins and ubiquinone-10 levels in patients with familial hypercholesterolemia
Hiroshi Mabuchi,Toshihiro Haba,Ryozo Tatami,Susumu Miyamoto,Yasuyuki Sakai,Takanobu Wakasugi,Akira Watanabe,Junji Koizumi,Ryoyu Takeda +8 more
TL;DR: It is concluded that ML-236B is effective in lowering serum cholesterol without lowering serum ubiquinone-10 in heterozygous patients with familial hypercholesterolemia.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme a reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity
TL;DR: The experiments reported in this paper demonstrate that MG236A and ML-236B inhibit specifically 3-hydroxy-3-methylglutaryl (HMG)CoA reductase (EC 1 .I .1.34), the rate-limiting enzyme in cholesterol synthetic pathway, without affecting the rest of the enzymes involved in this pathway, and that the inhibition is competitive with respect to the substrate HMG-CoA.
Book ChapterDOI
Regulation of HMG-CoA Reductase
TL;DR: This chapter discusses the regulation of HMG-CoA reductase, which catalyzes the rate-limiting reaction of hepatic sterol synthesis, which is an autosomal, dominant genetic disorder estimated to affect 0.1–0.2% of the population.