Synthetic enzyme inhibitors as antifertility agents
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TLDR
It is concluded that synthetic trypsin inhibitors successfully prevent fertilization in vivo and reduce the fertility of ejaculated sperm.About:
This article is published in FEBS Letters.The article was published on 1970-12-18 and is currently open access. It has received 75 citations till now. The article focuses on the topics: Acrosin & Proteolytic enzymes.read more
Citations
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An electron microscopic study of sperm penetration into the rabbit egg after natural mating.
TL;DR: The ultrastructure of fertilization has been studied in rabbit eggs recovered 11 to 15 hours after natural mating, finding that intact sperm were present close to the zona pellucida, and the mainpiece sometimes becomes fused with surface processes before it enters the body of the egg.
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Boar proacrosin. Purification and preliminary activation studies of proacrosin isolated from ejaculated boar sperm
K L Polakoski,R F Parrish +1 more
TL;DR: Two forms of proacrosin have been purified from ejacualted boar spermatozoa using benzamidine to inhibit the premeture activation of the zymogen and included pH precipitation, ammonium sulfate fractionation, and sodium chloride precipitation.
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p-Aminobenzamidine, an acrosin inhibitor, inhibits mouse sperm penetration of the zona pellucida but not the acrosome reaction.
TL;DR: Results are consistent, therefore, with a role for the acrosomal enzyme acrosin in matrix dispersal, but not the Acrosome reaction itself, and in penetration of the zona pellucida.
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Biochemistry of natural proteinase inhibitors.
TL;DR: Early X-ray structural data for an inhibitor and its enzyme complex provide an insight into the molecular structure of the latter and the interactions involved in the association to form the complex.
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Acrosomal Proteinase and Proteinase Inhibitor of Human Spermatozoa
TL;DR: The acrosomal proteinase of human spermatozoa was characterized and differs from other human proteinases, which has optimal activity at pH 8.0, and possesses a proteinase inhibitor that is similar to one of the proteinase inhibitors from human seminal plasma.
References
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Identification and subcellular localization of the enzymes effecting penetration of the zona pellucida by rabbit spermatozoa.
Richard Stambaugh,Jean Buckley +1 more
TL;DR: A sucrose density gradient centrifugation procedure for separating the subcellular components of washed and sonicated rabbit epididymal spermatozoa has been developed and applied to the sub cellular localization of several sperm enzymes, including the acrosomal enzymes involved in penetration of the zona pellucida by the spermatozoon.
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Inhibition of in Vitro Fertilization of Rabbit Ova by Trypsin Inhibitors
TL;DR: It is indicated that the acrosomal enzyme is the essential enzyme for penetration of the zona pellicida of the rabbit ovum and inhibition of in vitro fertilization.
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Relationship of a trypsin-like enzyme in rabbit spermatozoa to capacitation.
TL;DR: The existence of a trypsin-like enzyme (TLE) in acrosomes ofepididymal spermatozoa was confirmed and was further demonstrated to be present in acosomes of ejaculated and capacitated spermatozosa.
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A Sperm Enzyme That Disperses the Corona Radiata and Its Inhibition by Decapacitation Factor
TL;DR: Seminal plasma and partially purified decapacitation factor (DF), a substance from seminal plasma that renders sperm incapable of penetrating ova, inhibited CPE activity but not hyaluronidase or TLE, and Acid-inactivated DF and uterine fluids did not inhibit CPE.
Related Papers (5)
Inhibition of fertilization in vivo by pancreatic and seminal plasma trypsin inhibitors
Inhibition of in Vitro Fertilization of Rabbit Ova by Trypsin Inhibitors
Identification and subcellular localization of the enzymes effecting penetration of the zona pellucida by rabbit spermatozoa.
Richard Stambaugh,Jean Buckley +1 more