Journal ArticleDOI
Technology trends in antibody purification.
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TLDR
Technology trends in antibody purification are reviewed, including recent innovations in size exclusion, anion exchange, cation exchange, hydrophobic interaction, immobilized metal affinity, mixed-mode, and bioaffinity chromatography.About:
This article is published in Journal of Chromatography A.The article was published on 2012-01-20. It has received 264 citations till now.read more
Citations
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Aqueous two-phase systems for protein separation: phase separation and applications.
TL;DR: This paper analyses and reviews some elements that are important for implementation of these processes which are related to phase separation and continuous processing of ATPS.
Journal ArticleDOI
Trends in Upstream and Downstream Process Development for Antibody Manufacturing
TL;DR: This review presents an overview of existing methods for process optimization and integration and indicates new approaches for future developments.
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Continuous downstream processing of biopharmaceuticals
TL;DR: The problem of 'batch' definition has been solved and paved the way for implementation of continuous downstream processing of biopharmaceuticals from a regulatory viewpoint.
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Recent advances in bioprocessing application of membrane chromatography
TL;DR: This review discusses technological merits and disadvantages associated with membrane chromatography as well as recent bioseparation applications with a particular attention on purification of large biomolecules.
Journal ArticleDOI
Challenges of glycosylation analysis and control: an integrated approach to producing optimal and consistent therapeutic drugs.
Peiqing Zhang,Susanto Woen,Tianhua Wang,Brian Liau,Sophie Zhao,Chen Chen,Yuansheng Yang,Zhiwei Song,Mark R. Wormald,Chuanfei Yu,Pauline M. Rudd +10 more
TL;DR: The latest developments and challenges in glycosylation analysis and control in the context of bioprocessing monoclonal antibodies are reviewed.
References
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Journal ArticleDOI
Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution.
TL;DR: The model of human Fc fragment was refined at 2.9-A resolution and the R value of the model is 0.24, with strong arguments that contact 1 is the fragment B-Fc contact formed in solution under physiological conditions, while contact 2 is a crystal contact.
Journal ArticleDOI
Amino acid–base interactions: a three-dimensional analysis of protein–DNA interactions at an atomic level
TL;DR: The majority of amino acid-base interactions observed follow general principles that apply across all protein-DNA complexes, although there are individual exceptions.
Journal ArticleDOI
The isolation of IgG from mammalian sera with the aid of caprylic acid
M. Steinbuch,R. Audran +1 more
TL;DR: A technique is described for the precipitation of the bulk of the plasma proteins with caprylic (octanoic) acid without affecting IgG, ceruloplasmin, and part of the IgA.
Journal ArticleDOI
Downstream processing of monoclonal antibodies--application of platform approaches.
TL;DR: This paper describes the key elements of a flexible, generic downstream process platform for mAbs that has been adopted at Amgen and consists of a well-defined sequence of unit operations with most operating parameters being pre-defined and a small subset of parameters requiring development effort.
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