Journal ArticleDOI
Temperature influence on the dynamic binding capacity of a monolithic ion-exchange column.
Reads0
Chats0
TLDR
The binding capacity for both BSA and soybean trypsin inhibitor increased with increasing temperature and the effect on the dynamic binding capacity of dimers present in the BSA solution has been evaluated and a closed-loop frontal analysis was used to determine the equilibrium binding capacities.About:
This article is published in Journal of Chromatography A.The article was published on 2003-02-14. It has received 24 citations till now. The article focuses on the topics: Bovine serum albumin.read more
Citations
More filters
Journal ArticleDOI
Applications of polymethacrylate-based monoliths in high-performance liquid chromatography.
E. G. Vlakh,Tatiana B. Tennikova +1 more
TL;DR: In this review, the applications of polymethacrylate-based monolithic columns are summarized for the separation, purification and analysis of low and high molecular mass compounds in the different HPLC formats, including micro- and large-scale HPLC modes.
Book ChapterDOI
Convective Interaction Media (CIM)--short layer monolithic chromatographic stationary phases.
Aleš Podgornik,Aleš Štrancar +1 more
TL;DR: The purpose of this review is to give an overview about short methacrylate monolithic columns commercialised under the trademark Convective Interaction Media (CIM).
Journal ArticleDOI
Characterization of CIM monoliths as enzyme reactors.
TL;DR: The Michaelis-Menten constant K(m) and the turnover number k(3) of the immobilized enzymes were found to be flow-unaffected and indicate the absence of a diffusional limitation in immobilized CIM enzyme reactors.
Journal ArticleDOI
Modeling and robust pooling design of a preparative cation-exchange chromatography step for purification of monoclonal antibody monomer from aggregates.
Niklas Borg,Yan Brodsky,John Moscariello,Suresh Vunnum,Ganesh Vedantham,Karin Westerberg,Bernt Nilsson +6 more
TL;DR: A model that describes the separation of the monomer of monoclonal antibodies from the dimer and larger oligomers on preparative-scale using cation-exchange chromatography is implemented and used to establish an elution end collection criterion such that the step can robustly provide the target purity of monomers.
Journal ArticleDOI
Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules
TL;DR: The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through-put biomolecule purification on semi-preparative and preparative scales.
References
More filters
Book
Ion-Exchange Chromatography of Proteins
TL;DR: A 10-chapter book as mentioned in this paper is Volume 43 in the Chromatographic Science Series and includes information on theory, ion exchange equilibria, diffusion in the ion exchanger, factors affecting separation, and large-scale operation.
Journal ArticleDOI
Construction of large-volume monolithic columns.
TL;DR: A heat release during the polymerization is analyzed and a mathematical model is derived for the prediction of the maximal thickness of the monolithic annulus having a uniform structure for GMA-EDMA monoliths.
Journal ArticleDOI
Comparative studies on the isothermal characteristics of proteins adsorbed under batch equilibrium conditions to ion-exchange, immobilised metal ion affinity and dye affinity matrices with different ionic strength and temperature conditions.
TL;DR: The apparent changes in enthalpy and entropy for these protein-ligand interactions have been derived from the dependency of the change in the apparent Gibbs free energy on 1/T.
Journal ArticleDOI
Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns
TL;DR: In this article, the dynamic binding capacity of CIM® monolithic columns has been characterized for different linear velocities, up to a linear velocity of 2450 cm/h. Breakthrough experiments were performed for capacity determination.
Journal ArticleDOI
Isocratic separations on thin glycidyl methacrylate–ethylenedimethacrylate monoliths
TL;DR: In this article, the isocratic separation of oligonucleotides in the ion exchange mode on thin GMA-EDMA monoliths in the form of commercially available CIM (Convective interaction media) disks is presented.