Showing papers in "Journal of Chromatography B in 2003"
••
TL;DR: Four categories of protein precipitation techniques were tested in plasma using spectrophotometry to assess protein removal efficiency across a range of volumes, species and lots and acetonitrile, trichloroacetic acid and zinc sulfate were found to be optimal at removing protein in their categories.
613 citations
••
TL;DR: An isocratic reversed-phase HPLC method with ultraviolet detection with linear relationship obtained between the concentration and the detector signal and is applicable to rapidly and effectively analyze serum or plasma samples for therapeutic drug monitoring of about 30 antidepressants and atypical antipsychotics.
268 citations
••
TL;DR: This review article summarizes the recent progress on bioanalytical LC-MS/MS methods using underivatized silica columns and aqueous/organic mobile phases and proves the silica column demonstrated superior column stability.
259 citations
••
TL;DR: This review article gives an overview of derivatization methods of carbohydrates for their liquid chromatographic and electrophoretic separation, as well as the mass spectrometric characterization.
240 citations
••
TL;DR: The study can be useful as a guide for optimization of large-scale purification processes by determining equilibrium and dynamic binding capacity for human IgG and the capture of IgG from a crude feed-stock.
230 citations
••
TL;DR: The experimental determination of solute descriptors offers an alternative approach for estimating logP, and other biopartitioning properties, which is suitable for the rapid, high throughput determination of logP for neutral, weakly acidic, and weakly basic compounds.
225 citations
••
TL;DR: This review covers recent developments towards the implementation of multi-dimensional (MuD) liquid phase based systems for proteome investigations, and the emergence of approaches such as multiple HPLC-electrospray ionization tandem MS and capillary array electrophoresis-matrix assisted laser desorption ionization MS, have stimulated thinking towards developing a automated multi- dimensional system (MuDSy) that integrates liquid phasebased separation, digestion and identification of proteins in complex biological mixtures.
202 citations
••
TL;DR: A liquid chromatography-tandem mass spectrometry method is presented for the qualitative screening for 238 drugs in blood samples, which is considerably more than in previous methods.
174 citations
••
TL;DR: The selective grafting of activated methoxypoly(ethylene glycol) chains (with M(r) of 5000) on the free thiol functions of the proteinases provides an interesting alternative to the use of covalent and affinity chromatographies especially in the case of enzymes such as chymopapain that contains, in its native state, two thiol function.
171 citations
••
TL;DR: With this rapid and economic method the internal exposure of the general population to DEHP and other phthalates as well as the body burden of occupationally and medically exposed subjects can be determined.
168 citations
••
TL;DR: Two-dimensional-gel electrophoresis (2DE) and mass spectrometry (MS) have become widely used tools in the field of proteomics and protein and antibody microarrays have been developed as successor of DNA microarray to soon allow the proteome-wide screening of protein function in parallel.
••
TL;DR: A critical overview of the most significant methods available in the literature is presented and their value is discussed, especially with respect to their sensitivity and specificity.
••
TL;DR: Residues of malachite green were extracted from homogenized animal tissues with a mixture of McIlvaine buffer (pH 3.0)-acetonitrile, and purified over an aromatic sulfonic acid solid-phase extraction column followed by HPLC or LC-ESI-MS-MS analysis.
••
TL;DR: This improved method enabled us to analyze free un-conjugated mono-2-ethylhexyl phthalate (mEHP) by eliminating interferences derived from coelution of the glucuronide-bound, or conjugated form, of the mEHP on measurements of the free mE HP.
••
TL;DR: The current state of development in the area of attaching functionalized polymer brushes onto a microporous membrane to form a novel chromatographic medium for protein separation and purification is reviewed.
••
TL;DR: It is demonstrated that MTG can be used as a protein labeling reagent that is compatible with a subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF), and proposed thatMTG is a partially selective label towards some mitochondrial proteins.
••
TL;DR: The data presented in this report demonstrate that the method provides rapid, sensitive, precise and accurate measurements of curcumin concentrations in plasma and urine.
••
TL;DR: The pharmacokinetic profile indicates that ellagic acid has poor absorption and rapid elimination after oral administration pomegranate leaf extract, and part of it was absorbed from stomach.
••
TL;DR: In this paper, a high-performance liquid chromatographic (HPLC) method for the determination of polycyclic aromatic hydrocarbons (PAHs) in milk samples is described.
••
TL;DR: A sensitive and reproducible high-performance liquid chromatography (HPLC) method for measurement of plasma MDA in the assessment of lipid peroxidation is reported and recovery of MDA from its bound form is greatly improved.
••
TL;DR: The LC-EC method was the most sensitive, but CE-UV and CE-MS were sensitive enough for the determination of dopamine and methoxycatecholamines even in healthy patient urine.
••
TL;DR: The versatility of rational and combinatorial approaches suggests that, in the near future, they will become the dominant methods for designing and selection of novel affinity ligands with scale-up potential.
••
TL;DR: The present review gives a comprehensive overview over the current state-of-art of the technique, the available materials, important recent applications, as well as process design strategies and operating procedures.
••
TL;DR: It is concluded that antimicrobial hemoglobin-derived peptides could be important effectors of the innate immune response killing microbial invaders.
••
TL;DR: In the rat minocycline crossed the blood-brain barrier slowly, achieving mean brain concentrations between 30 and 40% of the equivalent systemic exposure, regardless of the dose and route of administration.
••
TL;DR: This review deals with proteins which have been used as immobilized chiral selectors for the enantioseparation of drugs in liquid chromatography and capillary electrophoresis, and chiral separations already achieved using immobilized protein selectors in affinity capillary electrochromatography (ACEC) are presented and compared in terms of efficiency, stability and reproducibility.
••
TL;DR: The technique of microdialysis is described and its application in pharmacokinetic studies, including the detailed surgical techniques in animal experiments from rat blood, brain, liver, bile duct and in vitro cell culture for unbound drug analysis is discussed.
••
TL;DR: A gas chromatographic-tandem mass spectrometric (GC-Tandem MS) method for the accurate quantification of ADMA in human plasma or serum and urine using de novo synthesized [2H(3)]-methyl ester ADMA (d(3)Me-ADMA) as the internal standard is described.
••
TL;DR: A new, fast, preparative and scaleable two-step chromatographic method for B-PE purification from the red microalga Porphyridium cruentum is presented, with a value significantly higher than those obtained following other methodologies.
••
TL;DR: The LC-MS assay, which proved superior because of its greater sensitivity and relatively short (7 min) run time, should be an important tool for future pharmacokinetic analyses of docetaxel and paclitaxel.