The Application of Pentoxifylline in the Stimulation of Sperm Motion in Men Undergoing Electroejaculation
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TLDR
These studies show that pentoxifylline stimulation can improve the movement characteristics of asthenospermic sperm from neurologically impaired men and may improve the chances for conception in some cases of male-factor infertility.Abstract:
The ability of pentoxifylline to stimulate the motion characteristics of antegrade and retrograde sperm collected at the time of electroejaculation with a rectal probe was assessed in six neurologically impaired men. Before electroejaculation, the bladder was rinsed and instilled with 20 to 30 ml Ham's F-10 medium. Washed sperm were incubated with various doses (0, 0.1, 1, and 3 mmol/L) of pentoxifylline. Video sequences were recorded at intervals from 0 to 4.5 hours and analyzed for sperm motion parameters using manual and computer-assisted semen analysis. The results were compared with equimolar concentrations of caffeine. Both pentoxifylline and caffeine demonstrated a dose-dependent stimulation of percent motility and other motion parameters. A maximal stimulation of two-fold to three-fold for both percent motility and curvilinear velocity, and 30% to 60% for straight line velocity was observed after incubation under these conditions. A significant increase in mean linearity was observed in samples incubated with 0.1 mmol/L pentoxifylline at 1.5 hours. Significant lateral head displacement was not observed at any time point. Two couples underwent gamete intrafallopian transfer (GIFT) in conjunction with this electroejaculation sperm stimulation procedure, and one has since delivered a normal child. These studies show that pentoxifylline stimulation can improve the movement characteristics of asthenospermic sperm from neurologically impaired men. Such sperm stimulation techniques do not affect the fertilization process and may improve the chances for conception in some cases of male-factor infertility.read more
Citations
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Journal ArticleDOI
Sperm preparation for ART.
Ralf Henkel,Wolf-Bernhard Schill +1 more
TL;DR: Sperm separation methods that yield a higher number of motile spermatozoa are glass wool filtration or density gradient centrifugation with different media and caffeine, pentoxifylline and 2-deoxyadenosine are substances that were used to stimulate motility.
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Role of oxidative stress and antioxidants in andrology and assisted reproductive technology.
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Effect of Cryoprotective Additives and Cryopreservation Protocol on Sperm Membrane Lipid Peroxidation and Recovery of Motile Human Sperm
TL;DR: The results support observations that there is a negative correlation between sperm motility and LPO in cryopreserved samples and discount the hypothesis that LPO protection is a result of the cryoprotective action of PAF or PTX.
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Cryopreservation of human spermatozoa with pentoxifylline improves the post-thaw agonist-induced acrosome reaction rate.
TL;DR: Treatment with pentoxifylline appears to minimize sperm damage during the freeze-thaw process and may improve fertilization rates with assisted reproductive procedures such as intrauterine insemination or in-vitro fertilization.
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Effects of phosphodiesterase-5 inhibitors on sperm parameters and fertilizing capacity.
Fotios Dimitriadis,Dimitrios Giannakis,N. Pardalidis,Kostas Zikopoulos,E. Paraskevaidis,N. Giotitsas,Valentina Kalaboki,Panagiota Tsounapi,Dimitrios Baltogiannis,Ioannis Georgiou,Motoaki Saito,T. Watanabe,Ikuo Miyagawa,Nikolaos Sofikitis +13 more
TL;DR: The effect that PDE5 inhibitors exert on sperm parameters may lead to the improvement of the outcome of assisted reproductive technology (ART) programs and in the future Pde5 inhibitors might serve as adjunct therapeutical agents for the alleviation of male infertility.
References
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Effects of phosphodiesterase inhibitors and cyclic nucleotides on sperm respiration and motility
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Fertility studies in the human male with traumatic injuries of the spinal cord and cauda equina.
TL;DR: The 18 cases studied in the present series were patients at Cushing Veterans Administration Hospital who had suffered traumatic injuries of the spinal cord and cauda equina, and the ages ranged from twenty-one to thirty-five years, with an average age of twenty-seven years.
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Sexual Dysfunction and Electroejaculation in Men with Spinal Cord Injury: Review
Carol J. Bennett,Carol J. Bennett,Stephen W.J. Seager,Stephen W.J. Seager,Elizabeth A. Vasher,Elizabeth A. Vasher,Edward J. McGuire,Edward J. McGuire +7 more
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Comparison of the effectiveness of placebo, clomiphene citrate, mesterolone, pentoxifylline, and testosterone rebound therapy for the treatment of idiopathic oligospermia
TL;DR: Treatment with the placebo, mesterolone, pentoxifylline, and testosterone rebound therapy did not result in a significant increase in the mean sperm concentration or pregnancy in the partners.
Journal ArticleDOI
Stimulation of ejaculated human spermatozoa by caffeine.
TL;DR: Caffeine (1, 3, 7-trimethyl-2, 6-dioxypurine), a cyclic nucleotide phosphodiesterase inhibitor, increased the motility of ejaculated human spermatozoa and the grade of motility or forward progression was increased in all groups up to one full unit.