The effects of microtubule stabilizing and recovery agents on vitrified bovine oocytes.
TLDR
In this paper , the effects of treatment with either a microtubule stabilizing agent, or a micro-tubule recovery agent were investigated in vitrified bovine oocytes.About:
This article is published in Theriogenology.The article was published on 2022-01-01 and is currently open access. It has received 3 citations till now. The article focuses on the topics: Medicine & Microtubule.read more
Citations
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Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes
TL;DR: In this paper , the effect of the vitrification of oocytes and embryos in assisted reproductive technology (ART) has been comprehensively summarized, and a review provides useful information for the safety assessment and further optimization of the current cryopreservation techniques in ART.
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Recovery of spindle morphology and mitochondrial function through extended culture after vitrification-warming of bovine oocytes.
TL;DR: In this article , the effects of vitrification on the meiotic spindle and mitochondrial function of bovine oocytes submitted to different times of post-warming culture were investigated.
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Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation
Daniel Angel-Velez,Tim Meese,Mohamed Hedia,A. Fernández-Montoro,T. de Coster,Osvaldo Américo Bogado Pascottini,Filip Van Nieuwerburgh,Jan Govaere,A. Van Soom,Krishna Chaitanya Pavani,Katrien Smits +10 more
TL;DR: In this article , the authors compared the transcriptome profiles of metaphase II equine oocytes vitrified before and after in vitro maturation, and found that the vitrification of in vitro matured oocytes presented subtle advantages in terms of the mRNA profile over the vitification of immature oocytes.
References
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Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
Takeshi Matsui,Mutsuki Amano,Takaharu Yamamoto,Kazuyasu Chihara,Masato Nakafuku,Mikako Ito,Takeshi Nakano,K. Okawa,A. Iwamatsu,Kozo Kaibuchi +9 more
TL;DR: P purified a Rho‐interacting protein with a molecular mass of approximately 164 kDa (p164) from bovine brain that bound to GTPgammaS (a non‐hydrolyzable GTP analog) and is likely to be a putative target serine/threonine kinase for Rho and serves as a mediator of the RHo‐dependent signaling pathway.
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Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification
Joseph Saragusty,Amir Arav +1 more
TL;DR: The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.
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The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: effect of β-mercaptoethanol, cysteine and cystine.
D.G. de Matos,C.C. Furnus +1 more
TL;DR: The high intracellular GSH levels after induction of GSH synthesis in bovine IVM by thiol compounds remain during IVF and are still present at the beginning of IVC, improving developmental rates.
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Taxol assembles tubulin in the absence of exogenous guanosine 5'-triphosphate or microtubule-associated proteins.
TL;DR: It is reported herein that taxol has the ability to promote microtubules assembly in the absence of microtubule-associated proteins, rings, and added guanosine 5'-triphosphate (GTP or organic buffer) and the drug enhances additional micro Tubulin assembly when added to microtubular assembly at apparent steady state.
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Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes
Rebecca R. Aman,John E. Parks +1 more
TL;DR: Spindles did not return to normal in most oocytes regardless of cooling temperature or rewarming scheme, and step-wise rewarming was no more beneficial than direct rewarming.