The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli.
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Although Pta- and Ack- mutants are greatly impaired in their growth on acetate, they incorporate [2-14C]acetate added to cultures growing on glycerol, but not on glucose; an inducible acetyl-CoA synthetase effects this uptake of acetate.Abstract:
Summary: Mutants of Escherichia coli
k12 have been isolated that grow on media containing pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mm-sodium fluoroacetate. Such mutants lack either acetate kinase [ATP:acetate phosphotransferase; EC 2.7.2.1] or phosphotransacetylase [acetyl-CoA:orthophosphate acetyltransferase; EC 2.3.1.8] activity. Unlike wild-type E. coli, phosphotransacetylase mutants do not excrete acetate when growing aerobically or anaerobically on glucose; their anaerobic growth on this sugar is slow. The genes that specify acetate kinase (ack) and phosphotransacetylase (pta) activities are cotransducible with each other and with purF and are thus located at about min 50 on the E. coli linkage map. Although Pta- and Ack- mutants are greatly impaired in their growth on acetate, they incorporate [2-14C]acetate added to cultures growing on glycerol, but not on glucose. An inducible acetyl-CoA synthetase [acetate:CoA ligase (AMP-forming); EC 6.2.1.1] effects this uptake of acetate.read more
Citations
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TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Journal ArticleDOI
A specific micromethod for the determination of acyl phosphates
TL;DR: In this communication, a method is described which utilizes the reaction of acyl phosphates with hydroxylamine and the acyl part of the acid anhydride is converted into hydroxamic acid.
Journal ArticleDOI
Recalibrated linkage map of Escherichia coli K-12.
B J Bachmann,K B Low,A L Taylor +2 more
TL;DR: This article corrects the article on p. 116 in vol.
Journal ArticleDOI
Enzymatic phosphorylation of acetate.
TL;DR: The metabolic utilization of acetate (oxidation via the citric acid cycle, synthesis of higher fatty acids, cholesterol, and possibly other compounds) requires a preliminary activation through conversion to acetyl C0A, and two main mechanisms are known to bring about this activation.