Showing papers in "Journal of Biotechnology in 2009"

TL;DR: The TFE strategy is an emerging technology aiming at enhancing the production of a particular metabolite by means of overexpressing TFs regulating the metabolic pathways involved in the accumulation of target metabolites and may avoid the inhibitive effects of the BE approaches and the limitation of "secondary bottlenecks" as commonly observed in the GE approaches.

TL;DR: Study of chloride- and acetate-based ILs for cellulose regeneration confirmed that all regenerated celluloses are less crystalline and more accessible to cellulase than untreated substrates and that cellulase is more thermally stable in the presence of Regenerated cellulose.

TL;DR: The genome of plant-associated Bacillus amyloliquefaciens FZB42 harbors an array of giant gene clusters involved in synthesis of lipopeptides and polyketides with antifungal, antibacterial and nematocidal activity and genes involved in ribosome-dependent synthesis of lantibiotics and other peptides are scarce.

TL;DR: The recent study regarding mushroom tyrosinase in the presence of ILs and their associated anions was used to illustrate the cooperative functioning of IL cations and anions in affecting enzyme performance, following the Hofmeister series.

TL;DR: Research and development activities at present include fundamentals of algae strain improvement, reactor design and process integration, with auxiliary power requirements and specific reactor cost being addressed as most critical issues.

TL;DR: This work experimentally determined metabolic fluxes in a tumor cell line, successfully recapitulating the hallmarks of cancer cell metabolism and identified the optimal tracer for analyzing individual fluxes, specific pathways, and central carbon metabolism as a whole.

TL;DR: The phylogenetic structure of the microbial community residing in a fermentation sample from a production-scale biogas plant fed with maize silage, green rye and liquid manure was analysed by an integrated approach using clone library sequences and metagenome sequence data obtained by 454-pyrosequencing.

TL;DR: It is shown here that the antibacterial polyketides difficidin and to a minor extent bacillaene act efficiently against E. amylovora.

TL;DR: In this review, the latest development of stress tolerance mechanisms was focused, and improvement of yeast stress tolerance by both random and rational tools was presented.

TL;DR: The recombinant E. coli strains - MG1655, DH5alpha, S17-1, XL1-Blue and BL21 - the DH5 alpha was found to be the best beta-carotene producer and glycerol as the carbon source for beta- carotene production was foundto be superior to glucose, galactose, xylose and maltose.

TL;DR: The phenotype of strain T7 may have important implications for biotechnological applications in which photosynthetic microalgae are used for large-scale culturing as an alternative plant biomass source.

TL;DR: This review integrates recent literature and the own data on the physiology of laccase and manganese peroxidase synthesis, focusing on the common characteristics and unique properties of individual fungi as well as on several approaches providing enhanced enzyme secretion.

TL;DR: expression analysis revealed that abscisic acid, salt, cold and osmotic stresses induce expression of LEA4-1 gene in leaf tissues in Brassica species, and results demonstrate that LEA 4-1 plays a crucial role in abiotic stress tolerance during vegetative stage of plant development.

TL;DR: A lipase from Thermomyces lanuginosus and cutinases from Thermobifida fusca and Fusarium solani hydrolysed poly(ethylene terephthalate) (PET) fabrics and films and bis(benzoyloxyethyl) tereplet (3PET) endo-wise as shown by MALDI-Tof-MS, LC-UVD/MS, cationic dyeing and XPS analysis.

TL;DR: A concept is proposed that processes wheat straw for ethanol and methane production by upgrading obtained ethanol to fuel-grade quality and providing methane to CHP plants for the production of electric energy and utility steam that is used to operate distillation columns in the ethanol production process.

TL;DR: An open source software pipeline, CLUSEAN (CLUster SEquence ANalyzer) that helps to annotate and analyze gene clusters of nonribosomal peptide synthetases/type I polyketide synthases and the prediction of specificities of NRPS.

TL;DR: In this article, mixed-mode ligands for protein purification have been reviewed and heterocyclic groups are good ligand candidates for their unique hydrophobicity and dissociation property.

TL;DR: Proof of concept that glycoengineered yeast can be used to produce functional full-length monoclonal antibodies at commercially viable productivities is provided.

TL;DR: It is shown that recombinant bacterial CotA-laccase from Bacillus subtilis is able to decolourise, at alkaline pH and in the absence of redox mediators, a variety of structurally different synthetic dyes.

TL;DR: It is shown that the ES cells can efficiently be encapsulated within the alginate beads, retaining a high level of cell viability, and the functionality of the encapsulated and differentiated cells was confirmed by their insulin production capability, whereby on glucose challenge the insulin production was found to be statistically significantly higher than for the cells from conventional two-dimensional differentiation system.

TL;DR: To increase the rate of HR events during fungal transformation, the orthologue of the human KU70 in H. jecorina tku70 was identified and deleted and provides a useful tool for a genome-wide functional gene analysis on a high-throughput scale to improve the biotechnological potential of this fungus.

TL;DR: The results of photo-response studies carried out on HRP/NanoCeO(2)/ITO bioelectrode indicate reasonable agreement with those obtained using amperometric technique.

TL;DR: An in silico model allows simulating optimization steps and provides a useful method to identify targets for directed genetic/physiological modification to optimize the system for a biotechnological approach.

TL;DR: Detailed results from the monitoring of two biogas plants and from the analysis of the separation of fermentation residues are presented, finding that the fermentation residues could be divided into 79.2% fluid phase and 20.8% solid phase, and the screw extractor separator exhibited a high throughput and good separation efficiency.

TL;DR: The potential of native silk sericin protein from silk gland as biocompatible biopolymer for potential biomedical applications is revealed and shows robustness, good mechanical strength and high temperature stability.

TL;DR: The most commonly used selection techniques for isolation of desired variants from large protein libraries are described and different antibody derivatives, as well as a variety of the most validated engineered protein scaffolds, are discussed.

TL;DR: Comparison of this innovative approach to traditional metagenomic-sequencing will illustrate that expression profiling has a promising future as a direct and very efficient method for the discovery of novel biocatalysts, even from complex microbial communities.

TL;DR: An IgG recovery yield of 89% and a protein purity of 75% can be achieved using a PEG/phosphate ATPS containing 10% NaCl, five stages and a volume ratio of 0.4, representing significant improvements in the recovery yield and purity when compared to a single-stage trial performed at the same experimental conditions.

TL;DR: A novel wheat-based bioprocess for the production of a nutrient-complete feedstock for the fermentative succinic acid production by Actinobacillus succinogenes has been developed and indicated that A. succ inogenes cells were able to utilise glucose and maltose in the wheat hydrolysate for cell growth and succinic Acid production.

TL;DR: Free and immobilized lipases from Candida antarctica, Thermomyces lanuginosus and Rhizomucor miehei were used as catalysts in the synthesis of butyl propionate by transesterification in reaction media consisting in nine different ionic liquids.