The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro
TLDR
A rapid method for measuring gas production during incubation of feedingstuffs with rumen liquor in vitro was described and gas production in 24 h from 200 mg feed dry matter was well correlated with digestibility of organic matter, determined in vivo with sheep.Abstract:
A rapid method for measuring gas production during incubation of feedingstuffs with rumen liquor in vitro is described. Gas production in 24 h from 200 mg feed dry matter was well correlated with digestibility of organic matter, determined in vivo with sheep. Multiple regression analysis, when it included data from proximate analysis, resulted in an equation ( R = 0·98) for prediction of metabolizable energy content, based on 30 experiments with rations varying in protein and crude fibre content, and 59 other experiments with concentrates. Energy content was in the range of 7·7–13·2 MJ ME/kg D.M. ( ± S.D. = 11·17 ± 1·08). The residual standard deviation of the equation was 0·25 MJ. Gas production was measured in calibrated syringes. The only chemical determinations needed are dry matter, protein and fat. Differences in activity between batches of rumen liquor are corrected by reference to gas production with standard feedingstuffs (hay meal and maize starch).read more
Citations
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Journal ArticleDOI
A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds.
TL;DR: Results obtained establish the pressure transducer as a suitable tool for determining the fermentation kinetics of ruminant feeds and ranking them with respect to their in vitro fermentability.
Journal ArticleDOI
In situ and Other Methods to Estimate Ruminal Protein and Energy Digestibility: A Review
TL;DR: This methodology offers a better way to simulate the rumen environment within a given feeding regimen, however, its usefulness will be dependent on the standardization of inherent variables associated with its conduct.
Book
Quantification of Tannins in Tree and Shrub Foliage: A Laboratory Manual
TL;DR: The first phase of the Co-Ordinated Research Project on Tannin Bioassays as mentioned in this paper was held in the early 1990s, and the results of the first phase were presented in the manual.
Journal ArticleDOI
Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques
TL;DR: The increase in gas production was associated with higher production of short-chain fatty acids with little change in their molar proportions, suggesting an increase in organic matter digestibility by inclusion of the PEG in tannin-rich feeds.
Journal ArticleDOI
Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle
M. Blu¨mmel,E. R. Ørskov +1 more
TL;DR: The Hohenheimer gas production test has been adapted to describe the kinetics of fermentation based on the exponential model p = a + b(1 − e−ct) as mentioned in this paper.
References
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Journal ArticleDOI
A two-stage technique for the in vitro digestion of forage crops
J. M. A. Tilley,R. A. Terry +1 more
TL;DR: A simple technique for the determination in vitro of the dry- or organic-matter digestibility of small (0·5 g) samples of dried forages is described, which involves incubation first with rumen liquor and then with acid pepsin.
Journal ArticleDOI
Determination of the balance of substances and calculation of the stochiometry and energy balance during in vitro fermentation of rumen fluid
K. H. Menke,Ursula Ehrensvärd +1 more
TL;DR: Es wird ein Verfahren zur In-vitro-Inkubation von Pansensaft beschrieben, das die Bestimmung von CO2, CH4, H2, fluchtigen Fettsauren, Ammoniak, Proteinen, Lipiden, Kohlenhydraten, Lignin and organischen Sauren in einer Analysenfolge ermoglicht.
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