Journal ArticleDOI
The Intracellular Accumulation of Metabolites by Ram Spermatozoa During Incubation in the Presence of Exogenous Fructose
RG Wales,EJ Humphries +1 more
TLDR
A method is described by which sufficient spermatozoa can be separated rapidly from their incubation medium to assay individual metabolites accumulating from [U·14C]fructose, and the main intracellular metabolite was Acetate.Abstract:
A method is described by which sufficient spermatozoa can be separated rapidly from their incubation medium to assay individual metabolites accumulating from [U·14C]fructose. Acetate and lactate made up 60-80% of the substrate carbon accumulated within the cell. Acetate was the main intracellular metabolite and its concentration in the cell was 10-20 times its extracellular level. The intra-cellular accumulation of lactate varied with the period of incubation but its concen-tration in the cell was always below that in the medium. The remaining substrate carbon in the cell was made up of fructose and at least three other unidentified compounds. No accumulation of substrate carbon was detected in citric acid cycle intermediates.read more
Citations
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Book ChapterDOI
Metabolism of D-fructose.
Minshen Chen,Roy L. Whistler +1 more
TL;DR: D-fructose exerts beneficial effects as a component of diets for mild and well-balanced diabetes, but should be taken within caloric restriction, as obesity impairs D-glucose tolerance and increases the insulin resistance of peripheral tissue.
Journal ArticleDOI
Some methods for washing spermatozoa from bull, boar and ram: a comparison using biochemical and other criteria.
R. A. P. Harrison,I. G. White +1 more
TL;DR: Evidence suggests that glucose-phosphate isomerase and hexokinase leak from the cytoplasmic droplets as well as from the spermatozoa during washing, and Hypo-osmotic media appear to cause an immediate and marked release of these enzymes from the droplets.
Journal ArticleDOI
Synthesis and metabolism of myoinositol in testicular and ejaculated spermatozoa of the ram
J. K. Voglmayr,I. G. White +1 more
TL;DR: It is suggested that the cytoplasmic droplet may be the site of inositol synthesis in the testicular spermatozoa.
Journal ArticleDOI
Pyruvate metabolism in boar spermatozoa.
D. E. Brooks,T. Mann +1 more
TL;DR: Pyruvate metabolism, in marked contrast to fructolysis, continues in spermatozoa immobilized by cold shock, and carbon dioxide, lactate, acetate, succinate and acetoacetate were identified as anaerobic products of pyruVate metabolism.
References
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Journal ArticleDOI
Effect of ions on the metabolism of ejaculated and epididymal ram spermatozoa
J. C. Wallace,R. G. Wales +1 more
TL;DR: There were differences between ejaculated and epididymal spermatozoa in the response of oxidative metabolism to potassium but, in general, the metabolic patterns of these cells were similar.
Journal ArticleDOI
Metabolism of sorbitol and fructose by ram spermatozoa
T. O'shea,R. G. Wales +1 more
Journal ArticleDOI
Metabolic End-products of Anaerobic Spermatozoan Metabolism
TL;DR: Results from many anaerobic metabolic investigations in the laboratory, however, in which bovine spermatozoa were incubated for 4 h at 37° C have consistently yielded low lactic acid values in relation to the fructose utilized.
Journal ArticleDOI
The Metabolism of Ram Spermatozoa in the Presence of Genital Fluids of the Ewe
RG Wales,BJ Restall +1 more
TL;DR: The metabolism of washed ram spermatozoa has been studied in the presence of fluids collected from the genital tract of the ewe, finding that vaginal, tubal, and follicular fluid stimulated aerobic and anaerobic glycolysis.
Journal ArticleDOI
The oxidative utilization of fructose and acetate by washed ram spermatozoa in the presence or absence of potassium and magnesium.
TL;DR: The oxidative utilization of fructose and acetate by washed ram spermatozoa has been studied during incubation in diluents containing varying levels of potassium and magnesium.