The Mechanism of Introduction of Alkyl Groups at C-24 of Sterols II. THE NECESSITY OF THE Δ24 BOND
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TLDR
A cell-free system capable of introducing 14C from methyl-labeled methionine into lanosterol, desmosterol, and cycloartenol was obtained in a supernatant from the centrifugation, confirming the earlier prediction that the Δ24(25) bond is necessary in order that alkylation may proceed.About:
This article is published in Journal of Biological Chemistry.The article was published on 1967-12-25 and is currently open access. It has received 44 citations till now. The article focuses on the topics: Lanosterol & Cycloartenol.read more
Citations
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Journal ArticleDOI
Role of sterols in membranes.
TL;DR: It is interpreted to mean that the primary role sterols play in nature is a nonmetabolic one as architectural components of membranes and that this role can be played, but less well, by other molecules which approximate the steroidal structure.
Journal ArticleDOI
The Biosynthesis of Ergosterol
TL;DR: The paper concentrates upon the post-squalene segment of the process as it occurs in fungi because the commercially important inhibitors of ergosterol biosynthesis are fungicides that block steps within that process.
Book ChapterDOI
The Biochemistry of Plant Sterols
TL;DR: The isolation and identification of a particular compound falls into the general realm of structural elucidation, which can become complicated, especially for an unusual sterol, however, for the common dominant structures, advances in chromatography and spectroscopy allow a simple procedure.
Journal ArticleDOI
Inhibition of S-adenosyl-L-methionine sterol-C-24-methyltransferase by analogues of a carbocationic ion high-energy intermediate. Structure activity relationships for C-25 heteroatoms (N, As, S) substituted triterpenoid derivatives.
TL;DR: The data obtained strongly support the idea that C-25 heteroatoms (N, As, and S), substituted triterpenoid derivatives possessing a positive charge at position 25, are analogues of a carbocationic high-energy intermediate involved during the reaction catalyzed by the AdoMet-cycloartenol-C-24-methyltransferase.
Journal ArticleDOI
Lanosterol biosynthesis in plants.
TL;DR: Phylogenetic reconstructions reveal that lanosterol synthases are broadly distributed in eudicots but evolved independently from those in animals and fungi, and novel catalytic motifs establish that plant lanosterl synthases comprise a third catalytically distinct class of lanosterols synthase.
References
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The biosynthesis of sterols in higher plants
LJ Goad,TW Goodwin +1 more
TL;DR: Under anaerobic conditions incorporation of [2-(14)C]-mevalonate into the non-saponifiable lipid of pea leaves was drastically decreased but radioactive squalene was accumulated and the significance of these results in connexion with phytosterol biosynthesis is discussed.
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Evidence for the Origin of the Ethyl Group of β-Sitosterol
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The analysis of mixtures of animal and vegetable fats. v. separation of sterol acetates by thin-layer chromatography in reversed-phase systems and on silica gel g-silver nitrate layers.
TL;DR: The above chromatographic analyses can be applied to the identification of unknown sterols isolated from natural sources, e.g. coconut fat and Asterina pectinifera.
Journal ArticleDOI
Zur Kenntnis der Triterpene. (87. Mitteilung). Über verschiedene Umwandlungsprodukte des Lanosterins
L. Ruzicka,Ed. Rey,A. C. Muhr +2 more