The noise of membrane capacitance measurements in the whole-cell recording configuration.
Peng Chen,Kevin D. Gillis +1 more
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TLDR
The results demonstrate that the optimal frequencies for capacitance measurements are higher than previously believed and it is demonstrated that the capacitance noise at high frequencies can be reduced by compensating for the voltage drop of the sine wave across the series resistance.About:
This article is published in Biophysical Journal.The article was published on 2000-10-01 and is currently open access. It has received 47 citations till now. The article focuses on the topics: Noise spectral density & Noise (radio).read more
Citations
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Biological and chemical sensors based on graphene materials
TL;DR: This article critically and comprehensively reviews the emerging graphene-based electrochemical sensors, electronic sensors, optical sensors, and nanopore sensors for biological or chemical detection and emphasizes on the underlying detection (or signal transduction) mechanisms.
Journal ArticleDOI
Cochlear Outer Hair Cell Motility
TL;DR: The functional and structural properties of prestin are described and whether outer hair cell motility might account for sound amplification at all frequencies is also a critical question and is reviewed here.
Journal ArticleDOI
Roles of Cholesterol in Vesicle Fusion and Motion
TL;DR: It is found that free cholesterol is able to directly trigger vesicle fusion, albeit with less potency and slower kinetics as compared to membrane depolarization stimulation, which underscores the versatile roles of cholesterol in facilitating exocytosis.
Journal ArticleDOI
Myosin II Activation and Actin Reorganization Regulate the Mode of Quantal Exocytosis in Mouse Adrenal Chromaffin Cells
TL;DR: An essential role for activity-evoked cytoskeletal rearrangement and the action of myosin II in the regulation of catecholamine and neuropeptide exocytosis is demonstrated and represents an essential element of the sympathetic stress response.
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Micro- and nanotechnologies for study of cell secretion
Yinxi Huang,Dong Cai,Peng Chen +2 more
TL;DR: This paper discusses conventional biochemical Assays and their Coupling with Microand Nanotechnologies, and other Techniques and Possibilities for Intracellular Interrogations.
References
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BookDOI
Single-channel recording
Bert Sakmann,Erwin Neher +1 more
TL;DR: A Practical Guide to Patch Clamping R.H. Penner, C.W. Heinemann, and P. Jonas.
Journal ArticleDOI
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
TL;DR: The size distribution of step-like capacitance changes is well compatible with the idea that steps of capacitance increase reflect individual events of exocytosis of chromaffin granules, whereas steps of the opposite polarity reflect the formation of vesicles or vacuoles by endocytotic processes.
Book ChapterDOI
Tight-Seal Whole-Cell Recording
Alain Marty,Erwin Neher +1 more
TL;DR: It is the experience that this way of penetrating a cell inflicts much less damage on the cell than the standard microelectrode impalement.
Journal ArticleDOI
Rates of diffusional exchange between small cells and a measuring patch pipette.
Michael Pusch,Erwin Neher +1 more
TL;DR: Fluorescent compounds and specific ion currents were used to study the kinetics of the diffusional exchange between small cells and patch pipettes in the tight seal whole cell configuration of the patch clamp technique and diffusion time constants were linearly related to the access resistance.
Journal ArticleDOI
Patch-clamp techniques for time-resolved capacitance measurements in single cells.
Manfred Lindau,Erwin Neher +1 more
TL;DR: Two methods are described for estimation of passive cell parameters such as membrane capacitance, membrane conductance and access resistance in tight-seal whole cell recording by using a time domain technique and a lock-in amplifier.
Related Papers (5)
Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.
Patch-clamp techniques for time-resolved capacitance measurements in single cells.
Manfred Lindau,Erwin Neher +1 more