Journal ArticleDOI
The nucleolar chromatin and the secondary constriction
Sibdas Ghosh,Neidhard Paweletz +1 more
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TLDR
The nucleolar chromatin is traced from early prophase to the metaphase stage, where in metaphase it is fully condensed and in mitotic chromosomes this chromatin remains surrounded by achromatic materials resembling the fibrillar centre.About:
This article is published in Cell Biology International Reports.The article was published on 1990-08-01. It has received 4 citations till now. The article focuses on the topics: Nucleolar chromatin & Prophase.read more
Citations
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Journal ArticleDOI
When rDNA transcription is arrested during mitosis, UBF is still associated with non-condensed rDNA.
TL;DR: Analysis of the three-dimensional distribution of the rDNA transcription factor, UBF, revealed that it was similar at each stage of mitosis in the secondary constriction, which argues in favor of the fact that mitotic inactivation is not the consequence of rDNA condensation.
Journal ArticleDOI
Relative distribution of rdna and proteins of the rna polymerase i transcription machinery at chromosomal nors
TL;DR: Using confocal and immunofluorescence microscopy, a model of NOR organization is proposed that ribosomal genes that were inactive in the preceding interphase would be present as condensed short Q-loops occupying the axial region of the NOR.
Journal ArticleDOI
Localization of chromatin in "persistent" nucleoli in okadaic acid treated HeLa cells.
TL;DR: In okadaic acid treated HeLa cells, the chromosomes sometimes condense without being accompanied by nuclear envelope breakdown, which strongly speak for the localization of nucleolar chromatin (ribosomal cistrons) within the dense nucleolar component of the interphase nucleolus.
Journal ArticleDOI
The linker histone H1.2 is a novel component of the nucleolar organizer regions
TL;DR: Results collectively suggest that NORs are independent structures and that the linker histone H1.2 is a novel component of this structure.
References
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Journal ArticleDOI
Visualization of Nucleolar Genes
O. L. Miller,Barbara R. Beatty +1 more
TL;DR: The presence of extrachromosomal nucleoli in amphibian oocytes has permitted isolation and electron microscopic observation of the genes coding for ribosomal RNA precursor molecules.
Book ChapterDOI
The nucleolar structure.
TL;DR: The nucleusolus is organized at the nucleolus-organizing region of the chromosomes, which are generally visible as secondary constriction regions in metaphase chromosomes, and the chromatin within the constriction region is lost at interphase inside the nucleoli.
Journal ArticleDOI
Localization of RNA polymerase I in interphase cells and mitotic chromosomes by light and electron microscopic immunocytochemistry
Ulrich Scheer,Kathleen M. Rose +1 more
TL;DR: It is concluded that rRNA genes loaded with polymerase I molecules are transmitted from one cell generation to the next one and that factors other than the polymerase itself are involved in the modulation of transcription of DNA containing r RNA genes during the cell cycle.
Book ChapterDOI
Cellular Sites of RNA Synthesis
TL;DR: The chapter discusses the more recent experimental work on the sites of RNA synthesis and the intracellular transport or redistribution of the RNA molecules, finding no evidence that RNA of these classes can undergo self-replication, within the cells, and all cytoplasmic RNA must be derived from the nucleus.
Journal ArticleDOI
Ultrastructural organization, sites of transcription and distribution of fibrillar centres in the nucleolus of the mouse oocyte.
TL;DR: Observations suggest that the rDNA, which is initially compacted in the primary fibrillar centre at the onset of nucleogenesis, progressively unravels and becomes distributed throughout the fibrillsar parts of the nucleolonema.