J
John W. Sedat
Researcher at University of California, San Francisco
Publications - 156
Citations - 22766
John W. Sedat is an academic researcher from University of California, San Francisco. The author has contributed to research in topics: Microscopy & Microscope. The author has an hindex of 72, co-authored 156 publications receiving 21557 citations. Previous affiliations of John W. Sedat include University of Florida & Lawrence Livermore National Laboratory.
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Journal ArticleDOI
Spatial partitioning of the regulatory landscape of the X-inactivation centre
Elphège P. Nora,Bryan R. Lajoie,Edda G. Schulz,Luca Giorgetti,Luca Giorgetti,Luca Giorgetti,Ikuhiro Okamoto,Ikuhiro Okamoto,Ikuhiro Okamoto,Nicolas Servant,Nicolas Servant,Nicolas Servant,Tristan Piolot,Tristan Piolot,Tristan Piolot,Nynke L. van Berkum,Johannes Meisig,John W. Sedat,Joost Gribnau,Emmanuel Barillot,Emmanuel Barillot,Emmanuel Barillot,Nils Blüthgen,Job Dekker,Edith Heard,Edith Heard,Edith Heard +26 more
TL;DR: In addition to uncovering a new principle of cis-regulatory architecture of mammalian chromosomes, this study sets the stage for the full genetic dissection of the mouse X-inactivation centre.
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Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination
Mats G. L. Gustafsson,Lin Shao,Peter M. Carlton,C. J. Rachel Wang,Inna N. Golubovskaya,W. Zacheus Cande,David A. Agard,David A. Agard,John W. Sedat +8 more
TL;DR: This work describes how spatially structured illumination microscopy can be applied in three dimensions to double the axial as well as the lateral resolution, with true optical sectioning, and has produced the first light microscopy images of the synaptonemal complex in which the lateral elements are clearly resolved.
Journal ArticleDOI
Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh,Peter M. Carlton,Sebastian Haase,Lin Shao,Lukman Winoto,Peter Kner,Brian Burke,M. Cristina Cardoso,David A. Agard,Mats G. L. Gustafsson,Heinrich Leonhardt,John W. Sedat +11 more
TL;DR: Three-dimensional structured illumination microscopy (3D-SIM) opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
Journal ArticleDOI
Polarization of Chemoattractant Receptor Signaling During Neutrophil Chemotaxis
TL;DR: Morphologic polarity is necessary for chemotaxis of mammalian cells and the pleckstrin homology domain of the AKT protein kinase, tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils to probe intracellular signals responsible for this asymmetry.
Journal ArticleDOI
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate
Haim Giloh,John W. Sedat +1 more
TL;DR: Longer photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.