Journal ArticleDOI
Visualization of Nucleolar Genes
O. L. Miller,Barbara R. Beatty +1 more
TLDR
The presence of extrachromosomal nucleoli in amphibian oocytes has permitted isolation and electron microscopic observation of the genes coding for ribosomal RNA precursor molecules.Abstract:
The presence of extrachromosomal nucleoli in amphibian oocytes has permitted isolation and electron microscopic observation of the genes coding for ribosomal RNA precursor molecules. Visualization of these genes is possible because many precursor molecules are simultaneously synthesized on each gene. Individual genes are separated by stretches of DNA that apparently are not transcribed at the time of synthesis of precursor rRNA in the extrachromosomal nucleoli.read more
Citations
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A census of human RNA-binding proteins.
TL;DR: This work presents a census of 1,542 manually curated RBPs that are analysed for their interactions with different classes of RNA, their evolutionary conservation, their abundance and their tissue-specific expression, a critical step towards the comprehensive characterization of proteins involved in human RNA metabolism.
Journal ArticleDOI
Spheroid Chromatin Units (ν Bodies)
Ada L. Olins,Donald E. Olins +1 more
TL;DR: Linear arrays of spherical chromatin particles about 70 angstroms in diameter have been observed in preparations of isolated eukaryotic nuclei swollen in water, centrifuged onto carbon films, and positively or negatively stained.
Journal ArticleDOI
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit
TL;DR: Electron microscopic and biochemical studies demonstrate that the fundamental structure of chromatin depleted of lysine-rich histones is composed of a flexible chain of spherical particles (nucleosomes), about 125 A in diameter, connected by DNA filaments, suggesting that histone F1 is involved in the superpacking of DNA in chromosomes and nuclei.
Journal ArticleDOI
A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis
François Dragon,Jennifer E. G. Gallagher,Patricia A. Compagnone-Post,Brianna M. Mitchell,Kara A. Porwancher,Karen A. Wehner,Steven Wormsley,Robert E. Settlage,Jeffrey Shabanowitz,Yvonne N. Osheim,Ann L. Beyer,Donald F. Hunt,Susan J. Baserga +12 more
TL;DR: A large ribonucleoprotein (RNP) complex from Saccharomyces cerevisiae that contains the U3 snoRNA and 28 proteins is purified and it is suggested that this complex may correspond to the terminal knobs present at the 5′ ends of nascent pre-rRNAs.
Journal ArticleDOI
Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae
TL;DR: Yeast ribosome biogenesis provide useful models for ribosomopathies, diseases in humans that result from failure to properly assemble ribosomes.
References
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Journal ArticleDOI
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
TL;DR: The ovarian DNA of Xenopus is described and it is demonstrated that it contains an excess of sequences coding for rRNA, which is particularly striking in ovaries of the toads Bufo and Xenopus, in which the differential synthesis occurs during pachytene.
Journal ArticleDOI
Properties and Composition of the Isolated Ribosomal DNA Satellite of Xenopus laevis
TL;DR: Isolated polycistronic ribosomal DNA of Xenopus laevis is internally heterogeneous in GC content, but contains few basic nucleotide sequences, which are integrated in the ribosomes together with DNA of high GC content.
Journal ArticleDOI
The binding of RNA polymerase to DNA.
TL;DR: The amount of RNA polymerase that can bind to DNA is measured by separating the enzyme bound to DNA from unbound enzyme by zone centrifugation, which shows that sRNA both charged and uncharged competes directly with DNA for the binding site on the enzyme.
Journal ArticleDOI
Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.
TL;DR: The nucleolus of eucaryotic cells has been identified by Perry and others'-4 as the site of ribosomal RNA synthesis and it has recently become possible to obtain from HeLa cells a nucleolar preparation which, as seen by electron microscopy, is relatively free of chromatin.
Journal ArticleDOI
RNA chain growth-rate in Escherichia coli
Hans Bremer,Dorothy Yuan +1 more
TL;DR: The theoretical expectation for the relation between the temporal changes in the molecular weight distribution of radioactively labeled RNA and the rate of RNA chain-growth has been calculated and is determined to be 55 nucleotides per second at 37 °C.
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