Journal ArticleDOI
The Quantification of Protein Amino Groups by the Trinitrobenzenesulfonic Acid Method: A Reexamination
Philippe Cayot,Gérard Tainturier +1 more
TLDR
The trinitrophenylation kinetic seems to be more dependent on protein structure than reactivity, and in evaluation of the available lysine in glycated proteins, the distinction between reductive alkylation and Maillard-type condensation necessitates quantitative evaluation of nonalkylated lysines in protein hydrolysate, compared to results with the TNBSA method.About:
This article is published in Analytical Biochemistry.The article was published on 1997-07-01. It has received 168 citations till now. The article focuses on the topics: Reagent & Hydrolysate.read more
Citations
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Journal ArticleDOI
The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development
Anna Sorushanova,Luis Delgado,Z Wu,Naledi Shologu,Aniket Kshirsagar,Rufus Raghunath,Anne Maria Mullen,Yves Bayon,Abhay Pandit,Michael Raghunath,Dimitrios I. Zeugolis +10 more
TL;DR: How advances in engineering, chemistry, and biology have enabled the development of bioactive, 3D structures that closely imitate native supramolecular assemblies and have the capacity to deliver in a localized and sustained manner viable cell populations and/or bioactive/therapeutic molecules is reviewed.
Journal ArticleDOI
Advanced glycation end products cause epithelial-myofibroblast transdifferentiation via the receptor for advanced glycation end products (RAGE)
Matthew D Oldfield,Leon A. Bach,Josephine M. Forbes,David J. Nikolic-Paterson,Anne McRobert,Vicki Thallas,Robert C. Atkins,Tanya M. Osicka,George Jerums,Mark E. Cooper +9 more
TL;DR: This study provides a novel mechanism to explain the development of tubulointerstitial disease in diabetic nephropathy and provides a new treatment target.
Journal ArticleDOI
Biodegradable amphiphilic poly(ethylene oxide)-block-polyesters with grafted polyamines as supramolecular nanocarriers for efficient siRNA delivery
TL;DR: The results of this study demonstrated the promise of novel amphiphilic PEO-b-P(CL-g-polyamine) block copolymers for efficient siRNA delivery and showed effective endosomal escape after cellular uptake.
Journal ArticleDOI
Measurement of 2-thiobarbituric acid values in dark chicken meat through derivative spectrophotometry: influence of various parameters.
TL;DR: Several variables (kind of filter paper, amount of sample, antioxidant addition, stability of the spectrophotometric measurement, and handling and storage of samples) were found to influence 2-thiobarbituric acid (TBA) values in dark chicken meat when an acid aqueous extraction method with derivativeSpectrophotometry was used.
Journal ArticleDOI
Nucleophilicities of amino acids and peptides
Frank Brotzel,Herbert Mayr +1 more
TL;DR: Nucleophilicity parameters N as defined by the correlation log k(20 degrees C) = s(N + E) have been determined in order to include amino acids into the most comprehensive nucleophile scales presently available, which provide a direct comparison of n-, pi-, and sigma-nucleophiles.
References
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Book
Advanced Organic Chemistry: Reactions, Mechanisms, and Structure
TL;DR: Localized Chemical bonding Delocalized Chemical Bonding Bonding Weaker than Covalent Stereochemistry Carbocations, Carbanions, Free Radicals, Carbenes and Nitrenes Mechanisms and Methods of Determining them Photochemistry Acids and Bases Effects of Structure on Reactivity Aliphatic Nucleophilic Substitution Aromatic Electrophilic Substitutes Aliphatically Electrophilic Substitution Free-Radical Substitution Addition to Carbon-Carbon Multiple Bonds Adding to Carbon Hetero Multiple Bonds Eliminations Rearrangements Ox
Journal ArticleDOI
Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System
TL;DR: A systematic study has been made of the separations of amino acids by elution analysis on columns of synthetic ion exchange resins, demonstrating that synthetic resins are capable of separating most of the common amino acids from one another.
Journal ArticleDOI
Photometric ninhydrin method for use in the chromatography of amino acids.
Stanford Moore,William H. Stein +1 more
TL;DR: In the present investigations of the chromatographic separation of amino acids, it has been observed that, when the color development is carried out in tubes exposed to the air, these difficulties appear to result primarily from the influence of dissolved oxygen.
Journal ArticleDOI
A modified ninhydrin reagent for the photometric determination of amino acids and related compounds
Stanford Moore,William H. Stein +1 more
TL;DR: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds and its application in drug discovery is described.
Book
Laboratory techniques in biochemistry and molecular biology
T. S. Work,E. Work +1 more
TL;DR: This book discusses laboratory techniques in biochemistry and molecular biology, as well as some of the applications of these techniques in medicine and food science.