The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate
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A protein comparison of the nodF protein showed it to be similar to the acyl‐carrier protein from Escherichia coli and barley, especially around the pantothenate‐binding region and on this basis it is thought that this protein may be involved in an acyl transfer reaction.Abstract:
The DNA sequence of ~3.5 kb of the nodulation (nod) region of the Rhizobium leguminosarum symbiotic plasmid pRL1JI was determined. Three open reading frames were identified; genes corresponding to these have been called nodD, nodE and nodF.nodD is adjacent to nodA and is transcribed in the opposite direction. The nodF and nodE genes are downstream of, and transcribed in the same direction as, nodD with 667 nucleotides between nodD and nodF and three nucleotides separating nodF and nodE. The induction of the nodFE operon requires the nodD gene product and a component present in plant root exudate. Regions of DNA sequence preceding nodF are similar to those preceding nodA; these sequences may be involved in the regulation of the expression of nodA and nodF. Analysis of nodD revealed an amino acid sequence similar to the predicted DNA-binding domain of known DNA-binding proteins. A protein comparison of the nodF protein showed it to be similar to the acyl-carrier protein from Escherichia coli and barley, especially around the pantothenate-binding region and on this basis it is thought that this protein may be involved in an acyl transfer reaction.read more
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Journal ArticleDOI
Molecular Basis of Symbiotic Promiscuity
TL;DR: It is suggested that restricted host ranges are limited to specific niches and represent specialization of widespread and more ancestral promiscuous symbioses.
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Rhizobium-legume nodulation: life together in the underground.
TL;DR: This review concerns a dramatic association, one of the few that has been studied in detail: the nitrogen fixing symbiosis between certain plants and microbes Rhizobium bacteria stimulate leguminous plants to develop root nodules, which the bacteria infect and inhabit.
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Molecular Genetics of Polyketides and Its Comparison to Fatty Acid Biosynthesis
TL;DR: The structure of the biotechnology of POL YKETIDE SYNTHESIS is studied to understand the role of polyketides in plant growth and their role in the environment.
Journal ArticleDOI
Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI
TL;DR: A region of 16.8 kb of the Sym(biosis) plasmid pRL1JI of Rhizobium leguminosarum, consisting of the established 9.7 kb nodulation region which confers nodulation ability on Vicia hirsuta and a region which appeared to be necessary for nodulation on V. sativa and Trifolium subterraneum, was subcloned as fragments in a newly developed IncQ transcriptional fusion vector.
Journal ArticleDOI
Developmental biology of legume nodulation
TL;DR: Many legumes respond to Rhizobium inoculation by developing unique structures known as nodules on their roots, and there is some controversy as to whether the legume nodule is an organ sui generis or a highly derived lateral root.
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TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
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