Journal ArticleDOI
The synthesis of antibacterial proteins in isolated fat body from cecropia silkmoth pupae.
Ingrid Faye,G. R. Wyatt +1 more
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Fat body from previously immunized diapausing pupae of the silkmoth, Hyalophora cecropia (Saturniidae), incubated in vitro, released antibacterial activity into the medium and incorporated 3H-leucine into the immunity proteins P1–P9.Abstract:
Fat body from previously immunized diapausing pupae of the silkmoth,Hyalophora cecropia (Saturniidae), incubated in vitro, released antibacterial activity into the medium and incorporated3H-leucine into the immunity proteins P1–P9. The release of antibacterial activity from fat body was also induced to some extent by injection of Ringer solution and, after 20 h in culture, by the injury of dissection.read more
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Book ChapterDOI
Invertebrate Immunity: Basic Concepts and Recent Advances
TL;DR: This chapter discusses basic concepts and advances of invertebrate immunity and provides a functional approach for blood cell classification and the cells are arranged into five main groups—namely, progenitor cells, phagocytic cells, hemostatic cells, nutritive cells, and pigmented cells.
Book ChapterDOI
Insect Haemolymph Proteins
TL;DR: The structure and function of major groups of hemolymph proteins that are common to all insects, storage proteins, lipoproteins, vitellogenins, and inducible antibacterial proteins are discussed.
Journal ArticleDOI
Cell-free immunity in insects
Hans G. Boman,Dan Hultmark +1 more
TL;DR: Sequence work shows that at least two of the cecropins originate from a gene duplication, which is a main part of the immune system of the Cecropia moth and several other insects.
Journal ArticleDOI
Cell-free immunity in Cecropia
TL;DR: Insects respond to live bacteria by the production of a potent antibacterial activity which is due to the synthesis of 15–20 immune proteins, which is taken advantage of both in the purification of 15 inducible immune proteins and in the isolation of immune RNA, used for the preparation of a cDNA library.
Journal ArticleDOI
Biochemical aspects of insect immunology
TL;DR: The defensive arsenal of insects, like that of man, contains both passive structural barriers against infection and a cascade of active responses to organ isms that gain access to the hemocoel following injury to the integument.
References
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Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Journal ArticleDOI
Insect Immunity I. Characteristics of an Inducible Cell-Free Antibacterial Reaction in Hemolymph of Samia cynthia Pupae
TL;DR: In this article, the authors found that the silk moth pupae of Samia cynthia contain an inducible antibacterial activity in their hemolymph, which was triggered by primary infection with either Escherichia coli K-12 or Enterobacter cloacae.
Journal ArticleDOI
The metabolism of silkmoth tissues. 1. Incorporation of leucine into protein
Edmund Stevenson,G.R. Wyatt +1 more
TL;DR: Fat body tissue from larvae and pupae of saturniid silkmoths was incubated with leucine-1-C14, and incorporation into total protein was measured, and the effect on amino acid incorporation was first evident in tissue taken 14 hr. after the injury and thereafter the activity declined toward the preinjury rate.
Journal ArticleDOI
Inorganic ion composition of haemolymph of the cecropia silkmoth: Changes with diet and ontogeny
TL;DR: Haemolymph from fifth instar Manduca sexta larvae reared on an artificial diet had ion levels very close to those in Cecropia similarly reared, but showed some quantitative differences from the other species.
Journal ArticleDOI
Insect immunity. 11. Simultaneous induction of antibacterial activity and selection synthesis of some hemolymph proteins in diapausing pupae of Hyalophora cecropia and Samia cynthia.
TL;DR: It is shown that pupae of the giant silkmoth Samia cynthia have a humoral antibacterial activity, which was formed simultaneously with a selective incorporation of amino acids into eight polypeptide chains characterized by their electrophoretic behavior.