Journal ArticleDOI
The synthesis of underhydroxylated collagen by 3T6 mouse fibroblasts in culture
TLDR
It is concluded that the 3T6 fibroblast can synthesize and secrete an underhydroxylated form of collagen, and that the hydroxylation process is highly sensitive to specific environmental factors which have no comparable effect on the synthesis of its substrate.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1972-04-15. It has received 58 citations till now. The article focuses on the topics: Hydroxyproline & Ascorbic acid.read more
Citations
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Regulation of collagen synthesis by ascorbic acid.
TL;DR: The results suggest that collage polypeptide synthesis, posttranslational hydroxylations, and activities of the twohydroxylases are independently regulated by ascorbate.
Journal ArticleDOI
The biosynthesis of collagen and its disorders (second of two parts).
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Stimulation of collagen gene expression by ascorbic acid in cultured human fibroblasts. A role for lipid peroxidation
TL;DR: Ascorbic acid induces lipid peroxidation and reactive aldehydes and this step may be necessary for the stimulation of collagen gene expression by ascorBic acid in cultured human fibroblasts.
References
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Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
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Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins.
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Modified procedure for the assay of H3- or C14-labeled hydroxyproline
Kale Juva,Darwin J. Prockop +1 more
TL;DR: Although the present procedure measures total radioactive hydroxyproline rather than the specific radioactivity of hydroxy Proline, it is more readily applicable to samples from a variety of sources.
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Chemistry of the collagen cross-links. Isolation and characterization of two intermediate intermolecular cross-links in collagen
TL;DR: The reduction of intact collagen fibrils with tritiated sodium borohydride was found to stabilize the aldehyde-mediated cross-links to acid hydrolysis and thus allowed their location and isolation from acid hydrolysates on an automatic amino acid analyser.