The use of equilibrium-density-gradient methods for the preparation and characterization of blood-group-specific glycoproteins
J. M. Creeth,M. A. Denborough +1 more
TLDR
The method of sedimentation equilibrium in a gradient of caesium chloride has been applied to the preparation of blood-group-specific glycoproteins from human ovarian-cyst fluids and it is shown that virtually complete separation from contaminating protein is easily accomplished in a single step.Abstract:
1. The method of sedimentation equilibrium in a gradient of caesium chloride has been applied to the preparation of blood-group-specific glycoproteins from human ovarian-cyst fluids: it is shown that virtually complete separation from contaminating protein is easily accomplished in a single step. 2. The glycoproteins isolated in this way have been characterized by analytical density-gradient experiments in both caesium chloride and caesium sulphate and values of the buoyant density, selective solvation and apparent molecular weight have been obtained. 3. In some cases, materials prepared from the same cysts by solvent extraction methods have also been characterized in these terms. 4. The selective solvation values are about 0.1 and 0.5g of water/g of glycoprotein in caesium chloride and caesium sulphate respectively. 5. The apparent molecular-weight values are much lower than the weight-average molecular weights, and it is shown that the origin of the discrepancy is heterogeneity in density of the glycoproteins. 6. Some sources of error in the interpretation of density-gradient schlieren patterns are examined.read more
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Journal ArticleDOI
Mucin-type glycoproteins.
Ger J. Strous,Jan Dekker +1 more
TL;DR: This review is designed to critically examine relations between structure and function of the different compounds categorized as mucin glycoproteins.
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Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica.
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Chemotactic behavior of Campylobacter jejuni.
M B Hugdahl,J T Beery,M P Doyle +2 more
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Book ChapterDOI
An Investigation of the I and dJ/da Concepts for Ductile Tearing Instability
TL;DR: In this article, the effect of compliance on the energy rate available and an appropriate estimation of work dissipation rate for the I theory were described and the dJ/da concept was used in both "exact" form and as originally stated in terms of T, with certain compliance terms modified.
Journal ArticleDOI
Properties of Gastric and Duodenal Mucus: Effect of Proteolysis, Disulfide Reduction, Bile, Acid, Ethanol, and Hypertonicity on Mucus Gel Structure
TL;DR: It is proposed in vivo that although adherent gastroduodenal mucus allows penetration of these agents to the underlying mucosa, it can remain in situ and continue to protect against acid (with HCO3-) and pepsin, thus minimizing mucosal damage and maximizing repair.