The utilization of RAPD-PCR for identifying thermophilic and mesophilic Bacillus species.
Reads0
Chats0
TLDR
A random amplified polymorphic DNA fingerprinting assay has been optimized that is able to discriminate between numerous thermophilic and mesophilic bacillus species and strains and may be a general way of improving the resolution of a RAPD protocol.Abstract:
A random amplified polymorphic DNA fingerprinting assay has been optimized that is able to discriminate between numerous thermophilic and mesophilic bacillus species and strains. Included in the analyses are thermophilic (able to grow at 55°C) strains of Bacillus stearothermophilus, B. kaustophilus, B. coagulans, B. sphaericus, B. thermodenitrificans, B. thermocatenulatus, B. thermoleovorans, B. licheniformis, B. brevis, B. thermoglucosidasius, B. caldolyticus, B. caldotenax, B. caldovelox, B. thermocloacae and B. smithii. Mesophilic strains of B. pumilus, B. subtilis, B. megaterium, B. circulans, B. cereus and B. mycoides can also be used for fingerprinting with the assay. Increasing the concentration of primer from 0.2 to 2.0 μM is shown to have a significant effect on increasing the number of amplification products that can be used for the discrimination or identification of individual strains or species. It is suggested that this may be a general way of improving the resolution of a RAPD protocol. The optimized conditions have been used successfully to trace B. stearothermophilus, B. licheniformis and other bacillus species and strains in an industrial setting.read more
Citations
More filters
Journal ArticleDOI
Thermophilic bacilli and their importance in dairy processing.
TL;DR: A greater understanding of the structure and composition of thermophilic bacilli biofilms within the context of the milk processing environment and their link with spore formation is needed to develop better control measures.
Journal ArticleDOI
Detection and impact of protease and lipase activities in milk and milk powders
TL;DR: In this paper, the authors focus on the characteristics of proteases (specifically proteinases) and lipases in milk and milk powders, detection methods for such enzymes and the effects of these enzymes on milk during storage.
Journal ArticleDOI
An evaluation of RAPD fragment reproducibility and nature
TL;DR: The data demonstrate that prudence should guide inferences about population structure and nucleotide divergence based on RAPD markers, and the nature of genetic variation uncovered by the RAPD method is unclear.
Journal ArticleDOI
Rope-Producing Strains of Bacillus spp. from Wheat Bread and Strategy for Their Control by Lactic Acid Bacteria
TL;DR: Two types of white wheat bread were investigated for rope spoilage and strains of lactic acid bacteria previously isolated from sourdough were first selected for antirope activity on bread slices and then used as starters for bread-making experiments.
References
More filters
Journal ArticleDOI
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Fingerprinting genomes using PCR with arbitrary primers
John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Book ChapterDOI
Genetic analysis using random amplified polymorphic DNA markers.
Journal ArticleDOI
Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting.
TL;DR: Within the DNA-oligonucleotide-DNA-polymerase-thermal cycler, reproducibility was excellent when the thermal cycler equipped with the best temperature regulation was used, but was not as good with another brand of thermalcycler.
Journal ArticleDOI
Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains.
TL;DR: Arbitrary primer polymerase chain reaction technology has been applied to the identification of commercial strains of Bacillus thuringiensis by using total DNAs extracted from single bacterial colonies as templates and should prove to be a powerful tool for identification and discrimination of individual B. thuringienne strains.