Journal ArticleDOI
Transformation ofLeuconostoc oenos by electroporation
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TLDR
An electroporation-induced transformation system was developed for Leuconostoc oenos and of the three plasmids tested, only pGK13 was expressed, and transformation efficiencies of 1 X 103 were obtained.Abstract:
An electroporation-induced transformation system was developed forLeuconostoc oenos. Of the three plasmids tested (pGK13, pAMS100 and pIL253), only pGK13 was expressed. Several parameters were studied with possible influence on transformation efficiency. Electroporation conditions of 2000 V/cm, 25μF and 200 ohm proved successful. Transformation efficiencies (number of cells/μg plasmid DNA) of 1 X 103 were obtained.read more
Citations
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Oenococcus oeni and malolactic fermentation – moving into the molecular arena
TL;DR: Malolactic fermentation is the bacterial-driven decarboxylation of L-malic acid to L-lactic acid and carbon dioxide, and brings about deacidification, flavour modifications and microbial stability of wine.
Journal ArticleDOI
Allelic Diversity and Population Structure in Oenococcus oeni as Determined from Sequence Analysis of Housekeeping Genes
TL;DR: This study constitutes the first step in the development of an MLST method for O. oeni and the first example of the application of MLST to a nonpathogenic food production bacteria.
Journal ArticleDOI
Genomic variations of Oenococcus oeni strains and the potential to impact on malolactic fermentation and aroma compounds in wine
TL;DR: A review of the links between O. oeni metabolism, genomic diversity and wine sensory attributes will explore the large genomic diversity within this species.
Journal ArticleDOI
Implications of new research and technologies for malolactic fermentation in wine.
TL;DR: This review summarises the latest research and technological advances in the rapidly evolving study of malolactic fermentation and investigates the directions that future research may take.
References
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Journal Article
Practical Implications of Malolactic Fermentation: A Review
TL;DR: In this article, the authors discuss the effect of malolactic fermentation on wine quality and methods currently available for its control, including stimulation of indigenous flora, inoculation of bacteria (either pure cultures or from wines already undergoing MLF), use of immobilized bacterial cells or enzymes, and practical measures to inhibit bacterial growth.
Journal Article
Occurrence and Growth of Lactic Acid Bacteria in Wine: A Review
TL;DR: The ecology, growth, and survival of lactic acid bacteria in wines along with the changes they cause to wine composition are discussed.
Journal ArticleDOI
Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium
TL;DR: The ability to transfer plasmid DNA via electroporation will greatly facilitate the application of recombinant DNA methodology and transposon technology to Gram‐positive bacteria for cloning and analysis of significant genes.
Journal ArticleDOI
Gaining access to the cytosol: the technique and some applications of electropermeabilization.
D E Knight,M C Scrutton +1 more
Journal ArticleDOI
A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation.
TL;DR: An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of StrePTococcus cremoris and Streptococcus lactis was performed and yielded transformant colonies in 14 to 24 h.