Open AccessJournal Article
Trichomonas vaginalis: repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis.
TLDR
It appears that the two set primers are highly specific of T. vaginalis and provide a useful tool for PCR diagnosis in asymptomatic and symptomatic patients especially among the HIV at risk individuals.Abstract:
Trichomoniasis is recognised as a major sexually transmitted disease (STD) in the world and may act as an acquired immunodeficiency syndromes (AIDS) co-factor by enhancing the transmission of human immunodeficiency virus (HIV). Diagnosis of Trichomonas vaginalis can be achieved by several methods, but sensitive detection means are still lacking. In this study a 2000-bp repeated DNA fragment of T. vaginalis was cloned. Part of a conserved region of this insert was sequenced, two primers (TVK3 and TVK4) were chosen and a highly sensitive detection by polymerase chain reaction (PCR) was then developed for T. vaginalis. All strains of T. vaginalis analysed with these primers gave the expected 350-bp fragment and a 450-bp additional fragment. Sequence analysis of these PCR amplification products revealed that the 450-bp fragment contained the 350-bp with a 100-bp insertion characterised by a TGG microsatellite. A second primer set, namely TVK3 and TVK7 (determined at the border of the insertion), yielded PCR products of expected sizes. After amplification we were able to detect a single parasite. We also detected T. vaginalis in vaginal fluids of patients with STD. There was no reaction with human DNA or other infectious agents. It appears that the two set primers are highly specific of T. vaginalis and provide a useful tool for PCR diagnosis in asymptomatic and symptomatic patients especially among the HIV at risk individuals.read more
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Biological and behavioural impact of an adolescent sexual health intervention in Tanzania: a community-randomized trial.
David Ross,John Changalucha,Angela Obasi,Jim Todd,Mary L. Plummer,Bernadette Cleophas-Mazige,Alessandra Anemona,Dean Everett,Helen A. Weiss,David Mabey,Heiner Grosskurth,Richard J. Hayes +11 more
TL;DR: The intervention substantially improved knowledge, reported attitudes and some reported sexual behaviours, especially in boys, but had no consistent impact on biological outcomes within the 3-year trial period.
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Trichomoniasis: clinical manifestations, diagnosis and management
TL;DR: Management of trichomoniasis is usually as part of a clinical syndrome; vaginal discharge for women and urethral discharge for men and a single dose of metronidazole is effective in the majority of cases.
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The prevalence of trichomoniasis in young adults in the United States.
William C. Miller,Heidi Swygard,Marcia M. Hobbs,Carol A. Ford,Mark S. Handcock,Martina Morris,John L. Schmitz,Myron S. Cohen,Kathleen Mullan Harris,J. Richard Udry,J. Richard Udry +10 more
TL;DR: It is found that trichomoniasis is moderately prevalent among the general U.S. population of young adults and disturbingly high among certain racial/ethnic groups.
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Prevalence, Incidence, Natural History, and Response to Treatment of Trichomonas vaginalis Infection among Adolescent Women
Barbara Van Der Pol,James A. Williams,Donald P. Orr,Byron E. Batteiger,J. Dennis Fortenberry +4 more
TL;DR: The incidence of T. vaginalis infection is high among adolescent women; untreated infections may last undetected for 3 months or longer; treatment with oral metronidazole is effective, and T. vaginais DNA disappears rapidly after treatment.
Journal ArticleDOI
Trichomonas vaginalis as a cause of urethritis in Malawian men.
Marcia M. Hobbs,Peter N. Kazembe,Andrea W. Reed,William C. Miller,Eniffa Nkata,Dickman Zimba,Celine Costello Daly,Hrishikesh Chakraborty,Myron S. Cohen,Irving F. Hoffman +9 more
TL;DR: In men with symptomatic urethritis, the median HIV RNA concentration in seminal plasma from men with T. vaginalis was significantly higher that in seminal Plasma from HIV-positive men without trichomonas, and there was no difference in the rate of HIV seropositivity in men with and without T. vaginais.