Open AccessJournal Article
Ultrastructural localization of non-specific alkaline phosphatase during cleavage and blastocyst formation in the mouse
Jacques Mulnard,Roger Huygens +1 more
TLDR
The localization of non-specific alkaline phosphatase activity during cleavage and blastocyst formation has been investigated in the mouse by electron microscopy and the significance of the differential localization of the enzyme is discussed, especially in relation to the differentiation mechanisms of the trophoblast and inner cell mass.Abstract:
The localization of non-specific alkaline phosphatase activity during cleavage and blastocyst formation has been investigated in the mouse by electron microscopy. The activityis detectable for the first time at the two-cell stage and is localized on the surface ofthe interblastomeric plasma membranes and on small cytoplasmic inclusions. It increases in the following stages, predominantly on the interblastomeric membranes, the outside membranes remaining devoid of reaction. From the four-cell stage on, small reactive grains are also observed in the crystalloid plates of the cytoplasm. At the morula stage, the plasma membranes of the inner mass cells are entirely marked by the reaction whereas the trophoblastic cells are polarized, with their inner surfaces positive and outside surfaces negative. At the blastocyst stage the enzyme is gradually eliminated from the membranes bordering the blastocoel and from the interblastomeric furrows of the trophoblast and primary endoderm. The significance of the differential localization of the enzyme is discussed, especially in relation to the differentiation mechanisms of the trophoblast and inner cell mass.read more
Citations
More filters
Journal ArticleDOI
The foundation of two distinct cell lineages within the mouse morula
Martin H. Johnson,C. A. Ziomek +1 more
TL;DR: It is suggested that the foundation of the trophectoderm and inner cell mass lineages lineages may occur by a process of differential inheritance, which supports the recently proposed polarization hypothesis.
Journal ArticleDOI
Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction
C. A. Ziomek,Martin H. Johnson +1 more
TL;DR: The development of the polarized surface binding of the fluoresceinated ligand concanavalin A (FITC-Con A) was studied in blastomeres of the early mouse embryo to find the development of surface polarity to be highly dependent upon cell contact.
Journal ArticleDOI
The calcium-dependent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development
TL;DR: It is found that ECCD-1 affects the pattern of polarization of the cell surface in late 8- and 16-cell-stage blastomeres, as detected by staining with fluorescence-labeled concanavalin A.
Journal ArticleDOI
From egg to epithelium.
Tom P. Fleming,Martin H. Johnson +1 more
TL;DR: The results allowed us to assess the importance of the chiral component of the Tournaisian skeleton, as well as the polypeptide-like properties of actin and actin-Associated proteins, in terms of their role in the construction of the tibia and skeleton.
Journal ArticleDOI
The molecular and cellular basis of preimplantation mouse development
TL;DR: Evidence is reviewed suggesting that maternal RNA transcripts made up to 20 days prior to ovulation are available for use after fertilization, that no significant KNA synthesis occurs between germinal vesicle breakdown and the mid‐2‐cell stage and that during this interval of transcriptional inactivity production of protein is regulated at a post‐transcriptional level.
References
More filters
Journal ArticleDOI
Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage.
TL;DR: Investigations by Seidel and Tarkowski have shown that although the majority of 1/2 blastomeres can regulate and develop into smaller but otherwise normal blastocysts, som e of them give rise to purely trophoblastic vesicles devoid of the inner cell mass.
Journal ArticleDOI
The new lead citrate method for the ultracytochemical demonstration of activity of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase).
TL;DR: The new method, utilizing “lead citrate” as capture reagent, for the ultracytochemical demonstration of non-specific alkaline phosphatase activity at high alkaline ranges of pH was introduced.
Journal ArticleDOI
An ultrastructural and cytological study of preimplantation development of the mouse.
TL;DR: Observations suggest that the contents of the Blastocoel may be derived from the cytoplasmic vesicles, which increase in number and size subsequent to fertilization and discharge their contents into the intercellular spaces; the blastocoel arises as these fluid-filled spaces become confluent and enlarge.
Journal ArticleDOI
Cell Surface Changes during Preimplantation Development in the Mouse
TL;DR: Scanning electron microscopy reveals microvilli on all preimplantation stages, indicates that their number and length may be dependent on embryo size, and provides examples of regional alterations in their number.
Journal ArticleDOI
Ultrastructural cytochemistry of membrane-bound phosphatases in preimplantation mouse embryos.
TL;DR: The time of appearance and the ultrastructural localization of the enzyme activity of alkaline phosphatase, 5′-nucleotidase, Mg 2+ -ATPase, transport ATPase, cyclic AMP phosphodiesterase, cAMP-PDase, and adenylate cyclase were investigated in unfertilized eggs and in mouse preimplantation embryos.
Related Papers (5)
Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction
C. A. Ziomek,Martin H. Johnson +1 more