Journal ArticleDOI
Use of reversed polarity and a pressure gradient in the analysis of disaccharide composition of heparin by capillary electrophoresis
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TLDR
A capillary electrophoresis method with reversed polarity, combining both the application of a voltage and a pressure gradient between the buffer vials, was developed for the analysis of eight heparin-derived delta-disaccharides obtained by enzymatic depolymerization.About:
This article is published in Journal of Chromatography A.The article was published on 1998-12-18. It has received 58 citations till now. The article focuses on the topics: Intestinal mucosa & Capillary electrophoresis.read more
Citations
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Journal ArticleDOI
Compositional analysis and quantification of heparin and heparan sulfate by electrospray ionization ion trap mass spectrometry.
Ola M. Saad,Julie A. Leary +1 more
TL;DR: A new method using a combination of electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectra (MSn) was developed for the identification and quantitative analysis of eight heparan sulfate (HS)- and heparin-derived delta-disaccharides obtained by enzymatic depolymerization, reducing sample consumption and analysis time of traditional methods.
Journal ArticleDOI
Recent developments in capillary electrophoresis and capillary electrochromatography of carbohydrate species
TL;DR: In this paper, the recent developments in capillary electrophoresis (CE) and capillary electrochromatography (CEC) of carbohydrates are discussed, and important applications are illustrated.
Journal ArticleDOI
Compositional profiling of heparin/heparan sulfate using mass spectrometry: assay for specificity of a novel extracellular human endosulfatase
TL;DR: A mass spectrometry-based method was developed and used to obtain unambiguous structural information on the glycosaminoglycans (GAGs) that comprise heparin/HS, and was applied to assay for theHeparin substrate specificity of a newly discovered human extracellular endosulfatase, HSulf-2, which has been implicated in tumorigenesis.
Journal ArticleDOI
Heparin Characterization: Challenges and Solutions
TL;DR: The structure-elucidation process, along with its challenges and opportunities for future analytical improvements, is reviewed and illustrated for a heparin-derived hexasaccharide.
Journal ArticleDOI
Advances in the separation, sensitive detection, and characterization of heparin and heparan sulfate
TL;DR: Recent developments in the analytical methods used for sensitive separation, detection, and structural characterization of heparin and HS are discussed.
References
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Book ChapterDOI
Structure and biological activity of heparin
TL;DR: The structure and biological activity of heparin are described, being an alternating copolymer of a hexosamine and an alduronic acid, and the repeating disaccharide units constituting the largest fraction of the most common GAG are elaborated in the chapter.
Book
Heparin : chemical and biological properties, clinical applications
David A. Lane,Ulf Lindahl +1 more
TL;DR: The history of heparin methods of structural analysis general physical properties chemical synthesis occurence and evolutionary aspects the mast cell system and the biochemistry, cell biology and pathophysiology of anticoagulant molecules of the vessel wall regulation of cellular proliferation.
Journal ArticleDOI
Examination of the substrate specificity of heparin and heparan sulfate lyases.
Robert J. Linhardt,Jeremy E. Turnbull,Hui Ming Wang,Duraikkannu Loganathan,John T. Gallagher +4 more
TL;DR: These enzymes showed activity against solitary glucuronate-containing disaccharides in otherwise highly sulfated domains including the saccharide sequence that contains the antithrombin binding region in heparin, and are powerful reagents for the structural/sequence analysis of hepar in and heparan sulfate.
Journal ArticleDOI
Purification and characterization of heparin lyases from Flavobacterium heparinum.
D L Lohse,Robert J. Linhardt +1 more
TL;DR: The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability, and their amino acid analyses and peptide maps demonstrate that while these proteins are different gene products they are closely related.
Journal ArticleDOI
Specificity studies on the heparin lyases from Flavobacterium heparinum
TL;DR: The high level of specificity of heparin lyase I makes it particularly suitable for use in the sequencing of he parin and heparan sulfate, while caution must be exercised in using heparIn lyases II and III to sequence hepar in and he paran sulfates because of their relatively broad specificity.